| 中国少鳞鳜不同群体mtDNA控制区序列的遗传变异分析 |
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| 引用本文： | 赵丽丽,赵金良.中国少鳞鳜不同群体mtDNA控制区序列的遗传变异分析[J].上海海洋大学学报,2007(5):409-413. |
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| 作者姓名： | 赵丽丽 赵金良 |
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| 作者单位： | 上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090 |
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| 基金项目： | 上海水产大学校科研和教改项目;上海市重点学科建设项目 |
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| 摘 要： | 少鳞鳜为东亚特有鱼类,为了解中国少鳞鳜在我国不同水域内的遗传结构特征,利用PCR扩增和直接测序法分析了长江、钱塘江、西江、南渡江34尾样品mtDNA控制区的序列差异。在长度838 bp的同源序列中,共发现81个变异位点,占分析位点总数的9.67%;定义了4个单倍型,每个群体仅发现一个单倍型,群体之间没有共享单倍型,且每个群体都有鉴别位点。群体间的核苷酸歧义度（Dxy）在0.360%-8.043%之间,南渡江群体与长江、钱塘江、西江3个群体间的差异最大。分子变异分析（AMOVA）得出群体间的遗传分化指数（Fst）为1.000（P〈0.001）,差异极显著。构建的NJ树中,4个群体分为两支,一支为南渡江群体,另一支为长江、钱塘江、西江群体。这些表明中国少鳞鳜群体的遗传多样性较低,不同地理群体之间出现了明显的遗传分化,南渡江群体与长江、钱塘江、西江3个群体之间的分化水平最高。
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| 关 键 词： | 中国少鳞鳜 群体 控制区 遗传变异 |
| 修稿时间： | 9/4/2006 12:00:00 AM |
Genetic variation of the mitochondrial DNA control region among 4 populations of Coreoperca whiteheadi |
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| ZHAO Li-li,ZHAO Jin-liang.Genetic variation of the mitochondrial DNA control region among 4 populations of Coreoperca whiteheadi[J].Journal of Shanghai Ocean University,2007(5):409-413. |
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| Authors: | ZHAO Li-li ZHAO Jin-liang |
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| Institution: | Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China |
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| Abstract: | Coreoperca is a group of freshwater fishes endemic in East Asia.To study the genetic structure and differentiation of C.whiteheadi in China,the sequences of mitochondrial DNA control region of 34 samples,collected from Yangtze River,Qiantangjiang River,Xijiang River and Nandujiang River,respectively,were analyzed by PCR and sequencing methods.Among the 838 bp length homologous sequences,81 variable sites(9.67%) were detected.4 haplotypes were identified,each belonged to one single population.No haplotype was shared among four populations while each population had some fixed diagnostic sites.The nucleotide divergence(Dxy) among populations was 0.360%-8.043%,while Nandujiang population showed significant divergence with the others.The fixation index(Fst) calculated by the analysis of molecular variance(AMOVA) reached 1.000(P<0.001).NJ tree of the control region suggested that the 4 populations were divided into two clades: one clade included Nandujiang population,another clade comprised Yangtze,Qiantangjiang,Xijiang populations.These results suggested that genetic variance of C.whiteheadi is relatively low and the genetic differentiation among the four populations is significant;the Nandujiang population diverged distinctly from the other three populations. |
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| Keywords: | Coreoperca whiteheadi population control region genetic differentiation |
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