| 蛋鸡J亚群禽白血病病毒HLJ09MDJ-1株的分离鉴定 |
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| 引用本文： | 潘伟,高玉龙,秦立廷,祁小乐,高宏雷,孙芬芬,王永强,王笑梅.蛋鸡J亚群禽白血病病毒HLJ09MDJ-1株的分离鉴定[J].动物医学进展,2010,31(11). |
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| 作者姓名： | 潘伟 高玉龙 秦立廷 祁小乐 高宏雷 孙芬芬 王永强 王笑梅 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,禽传染病研究室,黑龙江哈尔滨,150001 |
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| 基金项目： | 现代农业肉鸡产业技术体系建设项目，公益性行业(农业)科研专项项目 |
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| 摘 要： | 为了解蛋鸡中禽白血病当前流行毒株类型及其gp85基因分子特征,在临床症状和病理组织学观察的基础上,采用ELISA抗原检测、特异性PCR以及亚群特异性免疫荧光试验(IFA),证实从黑龙江省某鸡场送检蛋鸡中分离到1株J亚群禽白血病病毒(ALV-J),命名为HLJ09 MDJ-1;克隆了其gp85基因,并进行了序列分析比较。结果表明,该分离株的gp85基因与国内外其他ALV-J毒株的核苷酸同源性为88.2%～98.2%。其中,HLJ09 MDJ-1与英国原型株HPRS-103的同源性最高(98.2%),与美国株ADOL-7501的同源性最低(88.2%),表明HLJ09 MDJ-1的gp85基因与HPRS-103有较近的亲缘关系。本研究从蛋鸡中分离到ALV-J毒株,为研究ALV-J宿主嗜性改变和ALV-J对蛋鸡致病机制提供了素材。
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| 关 键 词： | 蛋鸡 J-亚群禽白血病病毒 分离鉴定 gp85基因 遗传进化分析 |
Isolation and Identification of Avian Leukosis Virus Subgroup J HJL09MDJ-1 Strain in Layers |
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| PAN Wei,GAO Yu-long,QIN Li-tin,QI Xiao-le,GAO Hong-lei,SUN Fen-fen,WANG Yong-qiang,WANG Xiao-mei.Isolation and Identification of Avian Leukosis Virus Subgroup J HJL09MDJ-1 Strain in Layers[J].Progress In Veterinary Medicine,2010,31(11). |
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| Authors: | PAN Wei GAO Yu-long QIN Li-tin QI Xiao-le GAO Hong-lei SUN Fen-fen WANG Yong-qiang WANG Xiao-mei |
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| Institution: | PAN Wei,GAO Yu-long,QIN Li-tin,QI Xiao-le,GAO Hong-lei,SUN Fen-fen,WANG Yong-qiang,WANG Xiao-mei(Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin,Heilongjiang,150001,China) |
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| Abstract: | To find out the current prevalent ALV strain in layers and its molecular characteristics,a strain of subgroup J avian leukosis virus(ALV-J),named HLJ09MDJ-1,was isolated and identified by clinical symptoms and histopathological observation,PCR amplication and immunofluorescence assay special to ALV-J from layers suspected with ALVs infection in a farm of Helongjiang province in China.Moreover,the gp85 gene of HLJ09MDJ-1 was cloned and analyzed with other ALV-J strains published in GenBank.The results showed... |
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| Keywords: | layer Avian leukosis virus subgroup J isolation and identification gp85 gene genetic analysis |
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