| 滁菊组织培养脱病毒技术研究 |
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| 引用本文： | 邓衍明,褚芳玲,华如枝.滁菊组织培养脱病毒技术研究[J].安徽农业科学,2007,35(14):4130-4130,4159. |
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| 作者姓名： | 邓衍明 褚芳玲 华如枝 |
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| 作者单位： | 安徽省滁州市农业科学研究所,安徽滁州,239000;安徽省滁州市农业科学研究所,安徽滁州,239000;安徽省滁州市农业科学研究所,安徽滁州,239000 |
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| 摘 要： | 采用改良热处理结合茎尖分生组织培养的方法,建立滁菊组培脱毒快繁技术体系.研究结果表明,滁菊茎段诱导的最佳培养基为MS+2.0 mg/L 6-BA+0.1 mg/L NAA;茎尖增殖的最佳培养基为MS+1.0mg/L 6-BA+0.1 mg/L NAA;壮苗生根的最佳培养基为1/2MS+0.1mg/L NAA.接种脱毒结果为,脱毒率＞83%,茎尖成活率＞42%.
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| 关 键 词： | 滁菊 组织培养 热处理 脱毒 |
| 文章编号： | 0517-6611（2007）14-04130-01 |
| 收稿时间： | 2007-02-13 |
| 修稿时间： | 2007-02-13 |
Study on Virus-free Technique of Chuzhou Chrysanthemum by Tissue Culture |
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| DENG Yan-ming et al.Study on Virus-free Technique of Chuzhou Chrysanthemum by Tissue Culture[J].Journal of Anhui Agricultural Sciences,2007,35(14):4130-4130,4159. |
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| Authors: | DENG Yan-ming |
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| Institution: | Agricultural Science Institute of Chuzhou, Chuzhou, Anhui 239000 |
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| Abstract: | Rapid propagation technical system of the virus-free plantlets of Chuzhou Chrysanthemum was established by shoot tip meristematic tissue culture combined with improved heating treatment.The optimum stem induction medium was MS 2.0 mg/L 6-BA 0.1 mg/L NAA.The optimum shoot tip propagation medium was MS 1.0 mg/L 6-BA 0.1 mg/L NAA.The optimum rooting medium was 1/2 MS 0.1 mg/L NAA.The virus-free ratio of shoot tips was above 83 % and the survival ratio was above 42 %. |
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| Keywords: | Chuzhou Chrysanthemum Tissue culture Heating treatment Virus-free |
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