| 仿刺参体腔细胞原代培养方法初探 |
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| 引用本文： | 迟刚,李强,王扬,李华.仿刺参体腔细胞原代培养方法初探[J].大连水产学院学报,2009,24(2):181-184. |
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| 作者姓名： | 迟刚 李强 王扬 李华 |
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| 作者单位： | 大连水产学院,农业部海洋水产增养殖学重点开放实验室,辽宁,大连,116023 |
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| 基金项目： | 国家科技支撑项目，辽宁省教育厅高等学校科研项目，大连水产学院校人才引进项目 |
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| 摘 要： | 以仿刺参Apostichopus japonicus体腔细胞为材料，分别用体腔液和MEM、L-15两种培养基进行原代培养，初步探讨了仿刺参体腔细胞培养的基本条件。结果表明：在18℃下封闭培养，体腔细胞在体腔液中直接培养能够贴壁、伸展，细胞呈球形和上皮样，但凝集现象严重；在合成培养基（MEM、L-15）中，体腔细胞凝集明显减少；与MEM培养基相比，体腔细胞在L-15培养基中贴壁时间长，伸展状态好，上皮样细胞较多，可培养至72h；培养基中血清的添加量与细胞的培养状态和可培养时间关系不明显。
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| 关 键 词： | 仿刺参 体腔细胞 原代培养 |
The primary culture methods of coelomocytes in sea cucumber Apostichopus japonicus |
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| CHI Gang,LI Qiang,WANG Yang,LI Hua.The primary culture methods of coelomocytes in sea cucumber Apostichopus japonicus[J].Journal of Dalian Fisheries University,2009,24(2):181-184. |
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| Authors: | CHI Gang LI Qiang WANG Yang LI Hua |
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| Institution: | CHI Gang,LI Qiang,WANG Yang,LI Hua(Key Laboratory of Mariculture,Agriculture Ministry,PRC,Dalian Fisheries Univ.,Dalian 116023,China) |
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| Abstract: | The coelomocytes in sea cucumber Apostichopus japonicus were primarily cultured in the sea cucumber coelomic fluid, MEM and L - 15 medium to evaluate the coelomocyte culture conditions. The results showed that the coelomocytes directly cultured in the coelomic fluid showed attachment and extension, with a strongly agglutination under closed culture at 18 ℃, and the cells were shaped like a sphere or skin. The coelomocytes cultured in the compound medium （ MEM, L - 15 ） showed less agglutination. The coelomocytes in L - 15 medium had a relatively long period of attachment and a better stretching fettle, and were cultured in 72 hours compared with MEM medium. There were no significant correlation between the culturing period and cell vitality and the serum concentration. |
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| Keywords: | Apostichopus japonicus coelomocyte primary culture |
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