| 利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性 |
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| 引用本文： | 陈红旗,陈宗祥,倪深,左示敏,潘学彪,朱旭东.利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性[J].中国水稻科学,2008,22(1):23-27. |
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| 作者姓名： | 陈红旗 陈宗祥 倪深 左示敏 潘学彪 朱旭东 |
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| 作者单位： | 1.中国水稻研究所 水稻生物学国家重点实验室， 浙江 杭州 310006； 2扬州大学 植物功能基因组学教育部重点实验室， 江苏 扬州 225009 |
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| 基金项目： | 国家自然科学基金专项基金资助项目（30623006）;浙江省自然科学基金资助项目（Y304187）;浙江省0406计划资助项目;长三角区域联合科技攻关资助项目. |
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| 摘 要： | 以C101LAC和C101A51为稻瘟病抗性基因的供体亲本，金23B为受体亲本，通过杂交、复交及一次回交，在分离世代，利用分子标记辅助选择技术结合特异稻瘟病菌株接种鉴定和农艺性状筛选，获得6个导入Pi 1、Pi 2和Pi 33基因的金23B导入系，其中导入系W1对稻瘟病的抗病频率为96.7%，明显高于携带单个基因的C104LAC（Pi 1）、C101A51（Pi 2）和北京糯（Pi 33）。基因聚合后抗病频率提高，说明基因聚合是培育稻瘟病持久抗性的有效方法之一。
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| 关 键 词： | 水稻 稻瘟病 抗病基因 分子标记辅助选择 |
| 文章编号： | 1001-7216（2008）01-0023-05 |
| 收稿时间： | 2007-03-02 |
| 修稿时间： | 2007-08-02 |
Pyramiding Three Genes with Resistance to Blast by Marker-Assisted Selection to Improve Rice Blast Resistance of Jin 23B |
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| CHEN Hong-qi,CHEN Zong-xiang,NI Shen,ZUO Shi-min,PAN Xue-biao,ZHU Xu-dong.Pyramiding Three Genes with Resistance to Blast by Marker-Assisted Selection to Improve Rice Blast Resistance of Jin 23B[J].Chinese Journal of Rice Science,2008,22(1):23-27. |
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| Authors: | CHEN Hong-qi CHEN Zong-xiang NI Shen ZUO Shi-min PAN Xue-biao ZHU Xu-dong |
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| Abstract: | Using rice lines C101LAC and C101A51 as the donors of the resistance genes to rice blast, three resistance genes (Pi 1, Pi 2 and Pi 33) were introgressed into an elite variety Jin 23B by crossing, backcrossing and multi crossing. Six near isogenic lines of Jin 23B with the three resistance genes were obtained through traditional breeding method combined with marker assisted selection method, and the resistance frequency was 96.7%. The results indicated that the resistance frequency of the lines with the three resistance genes were higher than that of single resistance gene line. It could be concluded that gene pyramiding is an effective approach for the improvement of rice varieties with durable resistance to rice blast. |
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| Keywords: | rice rice blast resistance gene marker-assisted selection |
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