| 鳙鱼维氏气单胞菌的分离鉴定及其毒力基因检测 |
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| 引用本文： | 朱若林,马腾,陈露,崔晓羽,张嫣然,孙创,彭开松,张晓华,鲍传和.鳙鱼维氏气单胞菌的分离鉴定及其毒力基因检测[J].安徽农业大学学报,2017,44(2):229-233. |
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| 作者姓名： | 朱若林 马腾 陈露 崔晓羽 张嫣然 孙创 彭开松 张晓华 鲍传和 |
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| 作者单位： | 安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036;安徽农业大学动物科技学院,合肥,230036 |
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| 基金项目： | 国家自然科学基金（31402331）, 安徽省现代农业产业技术体系(2016-2020)（皖农科[2016]84号）-水产产业体系, 安徽农业大学引进与稳定人才项目（YJ2015-15）和安徽农业大学校级大学生科技创新基金项目（XJ2015176） 共同资助。。 |
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| 摘 要： | 从患病鳙鱼中分离纯化得到一株优势菌株AH-YS,通过革兰氏染色、氧化酶试验、生化鉴定以及16S r RNA基因扩增、测序和系统进化分析等操作,结果显示,分离到的细菌为维氏气单胞菌。药敏试验显示,在使用的11种抗菌药物中,该菌株对氧氟沙星、诺氟沙星和恩诺沙星等9种药物敏感,对甲氧苄氨嘧啶和氨苄西林耐药。对9种毒力基因的扩增结果显示,可以检测到气溶素、细胞毒性肠毒素、鞭毛、弹性蛋白酶和酯酶5种。
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| 关 键 词： | NaCl胁迫 番茄 种子发芽 耐盐性 |
| 收稿时间： | 2016/10/9 0:00:00 |
Isolation, identification and virulence genes detection of Aeromonas veronii from Aristichthys nobilis |
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| ZHU Ruolin,MA Teng,CHEN Lu,CUI Xiaoyu,ZHANG Yanran,SUN Chuang,PENG Kaisong,ZHANG Xiaohua and BAO Chuanhe.Isolation, identification and virulence genes detection of Aeromonas veronii from Aristichthys nobilis[J].Journal of Anhui Agricultural University,2017,44(2):229-233. |
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| Authors: | ZHU Ruolin MA Teng CHEN Lu CUI Xiaoyu ZHANG Yanran SUN Chuang PENG Kaisong ZHANG Xiaohua and BAO Chuanhe |
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| Institution: | School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036,School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 and School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | A bacterial strain named AH-YS was isolated from diseased Aristichthys nobilis, which was identified as Aeromonas veronii using a combination of gram stain, oxidase test, biochemical identification, PCR amplification of 16S rRNA and phylogenetic analysis. Drug sensitive test of a total of 11 antimicrobial agents showed that AH-YS was sensitive to 9 drugs. PCR amplification of a total of 9 virulence genes revealed that 5 genes could be detected, which are aerolysin, cytotoxic enterotoxin, flagellin, elastase and lipase. |
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| Keywords: | Aeromonas veronii Aristichthys nobilis isolation and identification virulence genes |
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