| 利用蛋白质组学技术研究不同储藏条件稻谷陈化机制 |
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| 引用本文： | 孙术国,王若晖,林亲录,蒋春燕,丁玉琴,曹建中.利用蛋白质组学技术研究不同储藏条件稻谷陈化机制[J].农业工程学报,2017,33(18):277-284. |
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| 作者姓名： | 孙术国 王若晖 林亲录 蒋春燕 丁玉琴 曹建中 |
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| 作者单位： | 中南林业科技大学食品科学与工程学院/稻谷及副产物深加工国家工程实验室,长沙,410004 |
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| 基金项目： | 湖南省重点研发计划项目（2016NK2146）；国家自然科学基金面上项目（31571874）；长沙市科技计划重点项目（k1508009-21）。 |
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| 摘 要： | 为了研究储藏过程中不同温度和气调条件对稻谷品质劣化的影响,利用蛋白质组学技术探讨稻谷储藏陈化的分子机理,研究温度37℃、25℃和25℃+CO2气调下稻谷储藏90 d品质和蛋白质组的变化.结果表明,较37、25℃贮藏,25℃+CO2气调下稻谷储藏脂肪酸值升高最少,发芽率下降最少(P＜0.05).稻谷储藏产生125个差异蛋白点,其中37个蛋白得到鉴定,根据蛋白质的功能可分为5类,包括代谢(45.9％),细胞结构(29.7％),抗胁迫(2.7％),功能性蛋白(5.4％)和其他蛋白(16.3％).并鉴定出4个目标蛋白,分别为蛋白酶体亚基β-1(B26、D09和F16),葡糖-1-磷酸腺苷酰基转移酶(C01和E07),ADP-葡萄糖焦磷酸化酶大亚基(B04和F04)和乙酰辅酶A(A06和C05).采用蛋白质组学技术分析稻谷储藏过程中蛋白质组变化,结果表明高温储藏促进稻谷差异蛋白表达,CO2气调储藏可降低差异蛋白表达.对差异表达蛋白功能分析表明,稻谷陈化可能与糖代谢紊乱、蛋白质分解能力降低,抗氧化酶活性降低,脂肪水解和氧化增强有关.研究结果为稻谷的合理、安全储藏提供参考.
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| 关 键 词： | 蛋白 储藏 品质控制 蛋白质组学 陈化 稻谷 |
| 收稿时间： | 2017/6/19 0:00:00 |
| 修稿时间： | 2017/9/3 0:00:00 |
Molecular mechanism of rice aging during different storage conditions based on protein levels |
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| Sun Shuguo,Wang Ruohui,Lin Qinlu,Jiang Chunyan,Ding Yuqin and Cao Jianzhong.Molecular mechanism of rice aging during different storage conditions based on protein levels[J].Transactions of the Chinese Society of Agricultural Engineering,2017,33(18):277-284. |
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| Authors: | Sun Shuguo Wang Ruohui Lin Qinlu Jiang Chunyan Ding Yuqin and Cao Jianzhong |
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| Institution: | College of Food Science and Engineering, Central South University of Forestry and Technology/National Engineering Laboratory for Deep Processing of Rice and Byproducts, Changsha 410004, China,College of Food Science and Engineering, Central South University of Forestry and Technology/National Engineering Laboratory for Deep Processing of Rice and Byproducts, Changsha 410004, China,College of Food Science and Engineering, Central South University of Forestry and Technology/National Engineering Laboratory for Deep Processing of Rice and Byproducts, Changsha 410004, China,College of Food Science and Engineering, Central South University of Forestry and Technology/National Engineering Laboratory for Deep Processing of Rice and Byproducts, Changsha 410004, China,College of Food Science and Engineering, Central South University of Forestry and Technology/National Engineering Laboratory for Deep Processing of Rice and Byproducts, Changsha 410004, China and College of Food Science and Engineering, Central South University of Forestry and Technology/National Engineering Laboratory for Deep Processing of Rice and Byproducts, Changsha 410004, China |
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| Abstract: | Abstract: In this study, proteomics technology was used to study the molecular mechanism of rice grain stored under different conditions. The changes in the proteomic profiles of rice during storage at 37oC, 25oC, 25°C and CO2 controlled atmosphere were analyzed; the effects of temperature and CO2 controlled atmosphere storage on the quality of rice grain were also investigated. The results showed that a total of 125 differentially expressed proteins in the process of rice storage were detected and 37 protein spots were identified by mass spectrometry. According to the function of protein, the 37 identified proteins can be divided into five groups: metabolic (45.9%), cell structure (29.7%), stress (2.7%), functional protein (5.4%), and unclassified proteins (16.3%). We identified four groups of the target proteins, respectively expressed by the down-regulate proteasome subunit beta 1 (B26, D09 and F16), the up-regulate glucose-1-adenosine phosphate acyltransferase (C01 and E07), the down-regulate ADP-glucose pyrophosphorylase large subunit (B04 and F04), and the up-regulate acetyl coenzyme A (A06 and C05). The functional properties of these differentially expressed proteins and their essential role in plant metabolism were evaluated according to the bioinformatics method, combined with the analysis of changes in physicochemical properties of rice during storage under different conditions. The results indicated that high temperature storage led to a higher level of differential protein expression in rice. The appearance of numerous differential protein expressions might disturb glucose metabolism, which reduced the synthesis of the starch granule. In rice, high temperature storage resulted in a reduction of antioxidant activity of key enzymes and proteins and an increase of free fatty acid, the antioxidant activity played an important role in the prevention of protein, starch, and lipid oxidation and the reduction in the aging degree of rice. High temperature storage of rice might disturb protein metabolism and brought about a decrease in catabolism for protein degradation. The greater changes of protein expression, antioxidant activity, glucose metabolism, free fatty acid, and protein metabolism in rice at higher temperature might contribute to a fast deterioration of rice quality and a rapid aging of rice. Thus, functional analysis of these differentially expressed proteins showed that the high temperature promoted the differential protein expressions in rice at the molecular level, which could lead to the aging of the rice, and the mechanism of rice aging might involve glucose metabolism, protein decomposition, fat hydrolysis and oxidation. In our experiment, the surprising finding was that low-oxygen high-carbon dioxide controlled atmosphere storage of rice could effectively reduce the level of differential protein expression under high temperature conditions. Protein metabolism in rice stored during controlled atmosphere storage with carbon dioxide could be to a certain extent maintained at normal levels, disorders of glucose metabolism in rice was effectively suppressed by the low-oxygen high-carbon dioxide controlled atmosphere storage of rice. At the same time, the metabolism on the levels of starch synthesis tended to be normal and natural aging-related biochemical changes in rice were reduced, which thus delayed the process of quality deterioration in rice at high temperature. Our study provides new research ideas and methods for rice storage. |
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| Keywords: | proteins storage quality control proteomics aging rice |
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