| 三种体色黄鳝群体遗传多样性分析 |
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| 引用本文： | 凌俊,江河,胡玉婷,段国庆.三种体色黄鳝群体遗传多样性分析[J].水产学杂志,2016(4):6-10. |
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| 作者姓名： | 凌俊 江河 胡玉婷 段国庆 |
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| 作者单位： | 安徽省农业科学院水产研究所,安徽 合肥,230031 |
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| 基金项目： | 农业部行业(农业)科研专项(201003076);安徽省农科院黄鳝创新团队(12c0501);安徽省科技攻关项目(12010302056) |
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| 摘 要： | 利用线粒体Cyt b基因部分序列测序技术研究3种体色黄鳝Monopterus albus群体(深黄斑鳝、青黄斑鳝、青斑鳝)的遗传多样性与遗传差异。结果显示:三个群体黄鳝共有变异位点53个,单倍型36个;平均单倍型多样性分别为0.9153、0.7517、0.9457;平均核苷酸多样性分别为0.00426、0.00211、0.00601。群体间遗传分化指数(Fst)较小(0.00282~0.04970),分子方差分析(AMOVA)中仅有2.10%的变异来自群体间,表明三种不同体色黄鳝群体间未有明显遗传分化。群体遗传距离的聚类分析结果显示:深黄斑鳝与青黄斑鳝群体间亲缘关系最近,深黄斑鳝与青斑鳝亲缘关系最远。
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| 关 键 词： | 黄鳝 体色 细胞色素 b 遗传多样性 |
Genetic Diversity Analysis of Three Populations of Ricefield Eel Monopterus albus with Different Body Colors |
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| LING Jun;JIANG He;HU Yu-ting;DUAN Guo-qing.Genetic Diversity Analysis of Three Populations of Ricefield Eel Monopterus albus with Different Body Colors[J].Chinese Journal of Fisheries,2016(4):6-10. |
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| Authors: | LING Jun;JIANG He;HU Yu-ting;DUAN Guo-qing |
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| Institution: | LING Jun;JIANG He;HU Yu-ting;DUAN Guo-qing;Fishery Research Institute, Anhui Academy of Agricultural Sciences; |
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| Abstract: | The genetic diversity in three populations of ricefield eel Monopterus albus with deep yellow spot ( A), green yellow spot ( B), and green colors ( C) was investigated using mitochondrial Cyt b gene. 53 mutations of nucleotide acids and 36 haplotypes were observed in 82 samples, with average haplotype diversity of 0.9153 in population A, 0.7517 in population B, and 0.9457 in population C, and nucleotide diversity of 0.00426 in population A, 0.00211 in population B, and 0.00601 in population C. The molecular variance ( AMOVA) revealed that there was no significant genetic differentiation among the populations, with genetic differentiation index ( Fst) of 0.00282 ~ 0.04970. The cluster analysis based on genetic distance among populations indicated that population A and popula-tion B had the closest phylogenetic relationship, and population A and population C showed the farthest phylogenetic relationship. |
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| Keywords: | Monopterus albus color cytochrome b genetic diversity |
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