| 薄膜—均质法制备番茄红素脂质体的工艺研究 |
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| 引用本文： | 欧春凤,叶盛英,张静,韩笑.薄膜—均质法制备番茄红素脂质体的工艺研究[J].南方农业学报,2012,43(4):511-514. |
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| 作者姓名： | 欧春凤 叶盛英 张静 韩笑 |
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| 作者单位： | 华南农业大学 |
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| 基金项目： | 国家自然科学基金项目(30871446) |
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| 摘 要： | 【目的】优化制备番茄红素脂质体的生产工艺，提高包封率，减小颗粒粒径，最终提高番茄红素的生物利用度。【方法】采用薄膜—均质法制备番茄红素脂质体，通过单因素试验和正交试验，以包封率作为主要指标，筛选出最佳制备工艺（胆固醇—卵磷脂比、番茄红素—卵磷脂比、旋转蒸发温度、磷酸缓冲液pH），并观察脂质体形态和粒径特征。【结果】薄膜—均质法制备番茄红素脂质体的最佳工艺为: 胆固醇—卵磷脂比1∶3，番茄红素—卵磷脂比1∶20，旋转蒸发温度30℃，磷酸缓冲液pH 7.0。在最佳工艺条件下，番茄红素脂质体的包封率为71.65%，平均粒径840 nm。【结论】采用薄膜—均质法制备番茄红素脂质体具有工艺简单、重现性好，且脂质体包封率高、颗粒粒径小、稳定性较高等优点，可大规模用于实际生产。
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| 关 键 词： | 番茄红素 脂质体 薄膜—均质法 包封率 |
Preparation of lycopene liposome using membrane-homogeneous method |
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| OU Chun-feng,YE Sheng-ying,ZHANG Jing,HAN Xiao.Preparation of lycopene liposome using membrane-homogeneous method[J].Journal of Southern Agriculture,2012,43(4):511-514. |
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| Authors: | OU Chun-feng YE Sheng-ying ZHANG Jing HAN Xiao |
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| Institution: | (South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | Objective]The experiment was conducted to enhance the bioavailability of lycopene by improving entrapment rate and reducing particle size.Method]Lycopene liposome was prepared by membrane-homogeneous method.Entrapment rate was used as major index in single factor experiment and orthogonal test,where cholesterol/lecithin ratio,lycopene/lecithin ratio,rotating evaporation temperature and phosphate buffer solution were determined to obtain the optimal processing procedure.Furthermore,lipsome morphology and particle characteristics were recorded.Result]The best processing parameters were observed as:cholesterol/lecithin ratio of 1:3,lycopene/lecithin ratio of 1:20,rotating evaporation temperature of 30 ℃ and phosphate buffer solution pH 7.0.Under the best technological conditions,entrapment rate of lycopene was 71.65% and the average particle size was 840 nm.Conclusion]It was suggested that preparing lycopene lipsome using membrane-homogeneous method is simple and apt to control1.This method should be promoted in production due to its high entrapment rate,small particle and great stability. |
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| Keywords: | lycopene liposome membrane-homogeneous method entrapment rate |
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