| GA1-2菌株的分离鉴定及其对香蕉尖孢镰刀菌的抑菌效果 |
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| 引用本文： | 起登凤,邹良平,周登博,冯仁军,高祝芬,张锡炎.GA1-2菌株的分离鉴定及其对香蕉尖孢镰刀菌的抑菌效果[J].植物保护学报,2017,44(5):809-816. |
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| 作者姓名： | 起登凤 邹良平 周登博 冯仁军 高祝芬 张锡炎 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所, 海南 海口 571101,中国热带农业科学院热带生物技术研究所, 海南 海口 571101,中国热带农业科学院热带生物技术研究所, 海南 海口 571101,中国热带农业科学院热带生物技术研究所, 海南 海口 571101,中国热带农业科学院热带生物技术研究所, 海南 海口 571101,中国热带农业科学院热带生物技术研究所, 海南 海口 571101 |
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| 基金项目： | 国家现代农业（香蕉）产业技术体系（CARS-31），海南省科技合作专项（KJHZ2015-26） |
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| 摘 要： | 为了从土壤中分离筛选对香蕉尖孢镰刀菌具有良好拮抗作用的放线菌,采用平板稀释涂布法从四川省会理县干热河谷小麦根际土壤中进行放线菌分离,并采用平板对峙法和孢子萌发法进行筛选,通过形态特征、培养特征、生理生化特征以及16S r DNA序列分析对筛选菌株进行鉴定。结果表明,从四川省会理县干热河谷小麦根际土壤筛选获得一株对香蕉尖孢镰刀菌4号生理小种Fusarium oxysporum f.sp.cubense race 4(FOC4)菌丝和孢子萌发都有很强抑制作用的菌株GA1-2,对FOC4菌丝和孢子萌发抑制率分别为36.34%和94.81%。菌株GA1-2与薰衣草灰链轮丝菌Streptoverticillium lavenduligriseum的亲缘关系最近,相似率达99.85%,且其形态特征、培养特征、生理生化特征也与薰衣草灰链轮丝菌基本相符,因此将菌株GA1-2初步鉴定为薰衣草灰链轮丝菌。
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| 关 键 词： | 香蕉枯萎病 拮抗菌 薰衣草灰链轮丝菌 鉴定 |
| 收稿时间： | 2016/6/20 0:00:00 |
Isolation, identification of strain GA1-2 and its antifungal activity against Fusarium oxysporum f. sp. Cubense |
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| Qi Dengfeng,Zou Liangping,Zhou Dengbo,Feng Renjun,Gao Zhufeng and Zhang Xiyan.Isolation, identification of strain GA1-2 and its antifungal activity against Fusarium oxysporum f. sp. Cubense[J].Acta Phytophylacica Sinica,2017,44(5):809-816. |
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| Authors: | Qi Dengfeng Zou Liangping Zhou Dengbo Feng Renjun Gao Zhufeng and Zhang Xiyan |
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| Institution: | Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, China,Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, China,Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, China,Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, China,Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, China and Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan Province, China |
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| Abstract: | In order to screen antagonistic actinomyce strains with inhibitory activity against Fusarium oxysporum f. sp. cubense race 4 (FOC4) from the wheat rhizosphere soil in dry hot valley of Huili County in Sichuan Province, actinomycetes were isolated with pour plate method and confrontation culture and spore germination method. The results showed that a strain named GA1-2 was isolated by antagonistic experiments against FOC4. The strain GA1-2 had an inhibition rate of 36.34% and 94.81%, respectively, to the mycelial growth and spore germination of FOC4. 16S rDNA sequence analysis exhibited that the strain GA1-2 had the highest sequence similarity with Streptoverticillium lavenduligriseum (99.85%). Reconstruction of a phylogenetic tree revealed that the train GA1-2 had the closest genetic relationship with Stv. lavenduligriseum. Based on the similarity between the strain GA1-2 and Stv. lavenduligriseum in terms of morphological, cultural, physiological and biochemical characteristics and 16S rDNA sequence, the strain GA1-2 was identified as Stv. lavenduligriseum. |
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| Keywords: | banana wilt disease antagonistic strain Streptoverticillium lavenduligriseum identification |
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