| 池养罗氏沼虾生长缓慢原因初步分析 |
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| 引用本文： | 周俊名,戴习林,蒋飞,丁福江.池养罗氏沼虾生长缓慢原因初步分析[J].上海海洋大学学报,2017,26(6):853-861. |
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| 作者姓名： | 周俊名 戴习林 蒋飞 丁福江 |
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| 作者单位： | 上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学 水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海申漕特种水产开发公司, 上海 201516,上海申漕特种水产开发公司, 上海 201516 |
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| 基金项目： | 上海市虾类产业技术体系建设项目（沪农科产字[2014]第5号） |
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| 摘 要： | 通过调查分析池养罗氏沼虾的生长状况、主要病原感染情况、遗传多样性、水质以及感染WSSV罗氏沼虾生长存活试验,探讨池养罗氏沼虾生长缓慢原因。结果表明:2016年生长正常与生长欠佳两类池塘平均有效等位基因介于0.632 2~0.687 2之间,平均多态信息含量介于0.583 1~0.635 4之间,属于高度多态性,两种生长类型池塘罗氏沼虾各遗传参数指标和水质指标间均无显著性差异(P0.05);生长正常池塘罗氏沼虾在养殖50、100和150 d,体长、体质量等指标均显著高于生长欠佳池塘罗氏沼虾(P0.05),EHP、WSSV和IHHNV阳性检出率均显著低于生长欠佳池塘(P0.05),养殖220 d,两类池塘各生长状况指标无显著性差异(P0.05),两类池塘沼虾携带上述病原的阳性检出率显著高于前3次检疫结果(P0.05),同时生长正常池塘阳性检出率更高,雄虾数量更少,与2014、2015年调查塘干塘起捕前结果类似;人工感染WSSV罗氏沼虾,感染15、30和45 d,各浓度组生长状况指标存在显著性差异(P0.05),各生长状况指标均随着感染浓度的上升逐步降低。据此结合养殖中后期每隔10 d左右捕大留小的生产工艺,认为水质、种质差异或退化引起池塘罗氏沼虾生长缓慢可能性很小,而感染特定病原引起罗氏沼虾生长缓慢的可能性较大,且感染特定病原对雄虾生长的影响可能大于雌虾。
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| 关 键 词： | 罗氏沼虾 病原检测 遗传多样性分析 生长缓慢 白斑综合征病毒 |
| 收稿时间： | 2017/4/14 0:00:00 |
| 修稿时间： | 2017/5/18 0:00:00 |
The preliminary analysis of the reasons for the poor growth of Macrobrachium rosenbergii in pond |
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| ZHOU Junming,DAI Xilin,JIANG Fei and DING Fujiang.The preliminary analysis of the reasons for the poor growth of Macrobrachium rosenbergii in pond[J].Journal of Shanghai Ocean University,2017,26(6):853-861. |
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| Authors: | ZHOU Junming DAI Xilin JIANG Fei and DING Fujiang |
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| Institution: | Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China,Shanghai Shencao Special Fisheries Development Co., Shanghai 201516, China and Shanghai Shencao Special Fisheries Development Co., Shanghai 201516, China |
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| Abstract: | Through the investigation on the growth,pathogen detection,genetic diversity analysis,water quality detection and injecting WSSV to explore the poor growth of Macrobrachium rosenbergii.The results showed that SSR analysis:Mean He 0.632 2-0.687 2,Mean PIC 0.583 1-0.635 4,genetic diversity was high,and there was no significant difference in genetic index and water quality(P>0.05). The growth of normal growing pond was significantly better than that of poor growing pond in culture days(50 d, 100 d and 150 d) of Macrobrachium rosenbergii (P<0.05), while the positive rate of EHP, WSSV and IHHNV was significantly lower than that of poor growing pond (P<0.05). There was no significant difference in growth between the two groups for 220 d (P>0.05). The positive rate of pathogen was significantly higher than that of the previous three quarantine results (P<0.05). The positive rate of normal growing pond was higher, and the number of male shrimp of normal growing pond was less,and the results were in agreement with the results of the pathogen detection before the investigation in 2014,2015. By injecting WSSV for 15 d, 30 d and 45 d, there were significant differences in growth index of each concentration group (P<0.05), and each growth index decreased gradually with the increase of infection concentration. Therefore,combined with catching big and keeping small in the middle and late stages of culture in every 10 d,the germplasm degeneration and water quality do not affect the growth of Macrobrachium rosenbergii. Pathogen may affect the growth of Macrobrachium rosenbergii.The effects of pathogen on male are greater than on female. |
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| Keywords: | Macrobrachium rosenbergii pathogen detection genetic diversity analysis poor growth WSSV |
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