| 早竹丛枝病的调查及病原菌的分子鉴定 |
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| 引用本文： | 耿显胜,张威,仲建平,张守科,舒金平.早竹丛枝病的调查及病原菌的分子鉴定[J].林业科学研究,2017,30(5):805-811. |
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| 作者姓名： | 耿显胜 张威 仲建平 张守科 舒金平 |
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| 作者单位： | 中国林业科学研究院亚热带林业研究所, 浙江 杭州 311400,中国林业科学研究院亚热带林业研究所, 浙江 杭州 311400,浙江省德清县林业技术推广站, 浙江 德清 313200,中国林业科学研究院亚热带林业研究所, 浙江 杭州 311400,中国林业科学研究院亚热带林业研究所, 浙江 杭州 311400 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目（CAFYBB2014QA006）。 |
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| 摘 要： | 目的]对早竹丛枝病进行调查及病原菌分子鉴定,为早竹丛枝病的病害诊断和防治提供科学依据。方法]采用单株水平和单枝盘水平的2种病害分级标准对早竹丛枝病进行调查。使用植原体16S rDNA和真菌rDNA-ITS序列的特异性PCR引物,对早竹丛枝病的病原菌进行分子鉴定。结果]调查的6块样地早竹丛枝病的平均发病率为18.59%,平均病情指数为6.67。感病的早竹DNA样品能够扩增出真菌的rDNA-ITS序列,而不能够扩增出植原体的16S rDNA序列;扩增出的序列与报道的竹针孢座囊菌的序列同源性达到99.00%,与其它真菌的序列同源性最高仅为94.00%。结论]浙江省德清县早竹丛枝病的病原菌为竹针孢座囊菌。
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| 关 键 词： | 早竹 竹子丛枝病 竹针孢座囊菌 分子鉴定 病情指数 |
| 收稿时间： | 2016/12/15 0:00:00 |
Disease Severity Assessing and Molecular Identification of Pathogen Associated with Witches' Broom Disease of Phyllostachys praecox |
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| GENG Xian-sheng,ZHANG Wei,ZHONG Jian-ping,ZHANG Shou-ke and SHU Jin-ping.Disease Severity Assessing and Molecular Identification of Pathogen Associated with Witches' Broom Disease of Phyllostachys praecox[J].Forest Research,2017,30(5):805-811. |
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| Authors: | GENG Xian-sheng ZHANG Wei ZHONG Jian-ping ZHANG Shou-ke and SHU Jin-ping |
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| Institution: | Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China,Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China,Forestry Technology Extension Station of Deqing County, Zhejiang Province, Deqing 313200, Zhejiang, China,Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China and Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China |
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| Abstract: | Objective] In this study, the witches'' broom disease of Phyllostachys praecox was assessed, and the pathogen of the disease was identified through molecular biology techniques.Method] The disease severity was assessed by grading witches'' broom disease at individual level and branch level. The pathogen of witches'' broom disease of Ph. praecox was identified by specific PCR primers of phytoplasmal 16S rDNA and fungal rDNA-ITS sequences.Result] Disease assessment data showed that the average incidence of the 6 plots was 18.59%, and the average disease index was 6.67. PCR results indicated that all the samples infected were able to amplify rDNA-ITS sequence of fungus, and could not amplify 16S rDNA of phytoplasma. Comparative BLAST analysis determined that the amplicons shared 99.00% similarity with sequences from Aciculosporium take, and the highest similarity with other fungi was only 94.00%.Conclusion] A. take was the causal agent of witches'' broom disease of Ph. praecox of Deqing County, Zhejiang Province. |
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| Keywords: | Phyllostachys praecox bamboo witches'' broom Aciculosporium take molecular identification disease index |
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