| IGFBP5基因部分编码序列的SNP快速筛查及其突变对猪生产性能的影响 |
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| 引用本文： | 赵晓枫,欧阳克,张阳,徐宁迎.IGFBP5基因部分编码序列的SNP快速筛查及其突变对猪生产性能的影响[J].农业生物技术学报,2009,17(5):758-762. |
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| 作者姓名： | 赵晓枫 欧阳克 张阳 徐宁迎 |
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| 作者单位： | 浙江大学动物科学学院,杭州,310029 |
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| 基金项目： | 国家霞点基础研究发展规划(973)项目，国家高技术研究发展计划(86)项目 |
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| 摘 要： | 摘要：胰岛素样生长因子（IGFs）是一类重要的生长因子，具有促进细胞生长、分裂和分化的作用，而IGFBPs对IGFs的代谢与分布起着关键作用，研究发现IGFBPs有独立于IGF之外的生物活性，并且受到翻译后的多种修饰。本研究采用基因池PCR扩增直接测序法，快速寻找到了IGFBP5基因编码序列+534处SNP座位的G/A突变，以此建立了PCR-RFLP的基因分型方法。然后利用该方法检测了300头金华×皮特兰杂交F2代猪，结果显示有GG、GA、AA 3种基因型，它们的基因型频率分别为0.064、0.589和0.348， G基因频率为0.358，A基因频率为0.642，采用一般线性模型分析结果表明，IGFBP5不同的SNP基因型对断奶体重和肌肉系水力有不同的遗传效应（p<0.05）。
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| 关 键 词： | IGFBP5基因 基因池 SNP 断奶重 系水力 猪 |
| 收稿时间： | 2008-10-21 |
| 修稿时间： | 2008-12-26 |
Quick Screening SNP on Partial Coding Sequence of Insulin-like Growth Factor Binding Protein 5 Gene (IGFBP5) and Effect of Its Polymorphism on Production Performance in Pigs |
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| ZHAO Xiao-feng,OU Yang-ke,ZHANG Yang,XU Ning-ying.Quick Screening SNP on Partial Coding Sequence of Insulin-like Growth Factor Binding Protein 5 Gene (IGFBP5) and Effect of Its Polymorphism on Production Performance in Pigs[J].Journal of Agricultural Biotechnology,2009,17(5):758-762. |
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| Authors: | ZHAO Xiao-feng OU Yang-ke ZHANG Yang XU Ning-ying |
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| Institution: | ZHAO Xiao-feng,OU Yang-ke,ZHANG Yang,XU Ning-ying(College of Animal Science,Zhejiang University,Hangzhou 310029,China) |
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| Abstract: | Abstract: The insulin-like growth factors (IGFs) play a central role in cellar growth, division and differentiation, their binding proteins (IGFBPs) with various posttranslational modification are important for metabolism and distribution of IGFs, but their biological activity is independent from IGFs. In this study, one SNP mutation of G/A was rapidly screened by PCR with direct sequencing based on gene-pool in position +534 of IGFBP5 coding sequence, 300 F2 pigs of Jinhua×Pietrain crossbred was genotyped by PCR-RFLP. The results showed that three genotypes (GG, GA and AA) were found, the frequency of genotype GG, AG and AA were 0.064, 0.589 and 0.348 respectively, and the allele frequency of G and A was 0.358 and 0.642 respectively. The significant effect of various genotypes was found on weaning weight and water holding capacity by using the general linear model (p<0.05). |
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| Keywords: | SNP |
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