| 鸡腿菇中多酚氧化酶性质及其抑制剂研究 |
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| 引用本文： | 沈莉,朱凤妹,杜彬,张跃.鸡腿菇中多酚氧化酶性质及其抑制剂研究[J].安徽农业科学,2008,36(13):5402-5404. |
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| 作者姓名： | 沈莉 朱凤妹 杜彬 张跃 |
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| 作者单位： | 河北科技师范学院化学系,河北昌黎,066600;河北科技师范学院食品工程系,河北昌黎,066600;河北科技师范学院分析测试中心,河北昌黎,066600 |
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| 摘 要： | 目的]为鸡腿菇的保鲜提供理论依据。方法]从新鲜鸡腿菇中提取多酚氧化酶(PPO)的粗酶液,研究pH值和温度对鸡腿菇PPO活性的影响,分析其热稳定性,并探讨亚硫酸氢钠、抗坏血酸和柠檬酸3种抑制剂对鸡腿菇PPO活性的抑制作用。结果]在强酸性和强碱性环境中,PPO都会失去活性。PPO酶活性随保温时间的延长呈降低趋势,说明PPO是一种热敏感性酶。3种抑制剂对鸡腿菇PPO活性均有抑制作用,其中NaHSO3的抑制作用最强,抗坏血酸和柠檬酸次之。结论]在鸡腿菇的加工过程中,添加NaHSO3能有效降低PPO活性和防止褐变的发生。
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| 关 键 词： | 鸡腿菇 多酚氧化酶 性质 抑制剂 |
| 文章编号： | 0517-6611(2008)13-05402-02 |
| 修稿时间： | 2008年3月3日 |
Study on the Properties of Polyphenoloxidase from Coprinus comatus and Its Inhibitors |
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| SHEN Li.Study on the Properties of Polyphenoloxidase from Coprinus comatus and Its Inhibitors[J].Journal of Anhui Agricultural Sciences,2008,36(13):5402-5404. |
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| Authors: | SHEN Li |
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| Abstract: | Objective] The aim of the research was to provide the theoretical basis for the fresh-keeping of Coprinus comatus.Method] The crude enzyme liquid of polyphenoloxidase(PPO)was extracted from fresh C.comatus to study the effects of pH and temperature on PPO activity in C.comatus and analyze its thermal stability And the inhibitions of 3 kinds of inhibitors(sodium sulfite,ascorbic acid and citric acid) on PPO activity in C.comatus were discussed.Result] In strong acidic and strong alkaline circumstances,PPO lost its activity.The enzyme activity of PPO showed a decreasing trend with the prolonging of heat-preservation time,which indicated that PPO was a kind of thermal-susceptible enzyme.Three kinds of inhibitors all had inhition on the activity of PPO from C.comatus.Among them,the inhibition of NaHSO3 was strongest,followed by ascorbic acid and citric acid.Conclusion] In the processing course of C.comatus,adding NaHSO3 could effectively decrease PPO activity and prevent the occurrence of browning. |
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| Keywords: | Coprinus comatus Polyphenoloxidase Property Inhibitor |
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