| 南京地区肉鸽大肠杆菌分离与鉴定 |
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| 引用本文： | 方光远,;季凤娟,;胡志华,;陈俊红,;顾亚凤,;蒋加进.南京地区肉鸽大肠杆菌分离与鉴定[J].南京农专学报,2014(4):86-89. |
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| 作者姓名： | 方光远 ;季凤娟 ;胡志华 ;陈俊红 ;顾亚凤 ;蒋加进 |
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| 作者单位： | [1]金陵科技学院动物科学与技术学院,江苏南京210038; [2]江苏省南通市通州区二甲镇农业服务中心,江苏南通226321 |
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| 基金项目： | 南京市科技局项目资助（201301065）;南京市2012年科技发展指导性项目（2012ZD006） |
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| 摘 要： | 为了分析南京地区肉鸽场乳鸽死亡的原因,对7份死亡乳鸽病料进行细菌分离培养,获得了7株细菌。对这7株菌株进行了分离培养、染色镜检、生化特性鉴定、血清型鉴定以及药敏试验,结果表明：1）7株细菌均为大肠杆菌;2）7株细菌对14种药物中头孢呋辛、头孢曲松、头孢噻肟、多粘菌素B敏感,对氨苄西林、链霉素、丁胺卡那耐药率分别高达85.7%、71.4%和85.7%。此外,从这7株细菌中随机选取1株细菌进行16SrDNA基因片段测序,发现该细菌407bp核苷片段与GenBank中已知8株大肠杆菌对应核苷酸片段同源性均高达99%以上。
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| 关 键 词： | 肉鸽 大肠杆菌 分离与鉴定 |
The Isolation and Identification of Escherichia coli of Pigeon in Nanjing |
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| Institution: | FANG Guang-yuan ,JI Feng-juan , HU Zhi-hua ,CHEN Jun-hong ,GU Ya-feng ,JIANG Jia-jin (1.Jinling Institute of Technology, Nanjing 210038, China;2.Agricultural Service Center of Erjia town of Tongzhou o{ Nantong of Jiangsu, Nantong 226321, China) |
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| Abstract: | Seven strains of bacteria are isolated from seven tain the causes of dead squabs in pigeon farms in Nanjing. dead pigeon diseases in order to ob Seven bacteria are tested with cuhu ring characteristics, staining characteristics, biochemical properties, serotype identification and susceptibility.Results show that seven bacteria are all Escherichia coli. seven bacteria are sensitive to Cefuroxime,Ceftriaxone,Cefotaxime,Polymyxin B,and the rates of resistance to Ampicillin,Streptomycin,Amikacin in 14 drugs are 85.7%,71.4% and 85.7% respectively.One bacterium is randomly selected for sequencing 16SrDNA gene. Results show that the homologies are more than 99% between the bacteria 407 bp nucleotide fragments and eight known strains of Escherichia coli nueleotide fragments in GenBank. |
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| Keywords: | pigeon Escherichia coli isolation and identification |
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