| 云南省蝴蝶兰上凤仙花坏死斑病毒的鉴定 |
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| 引用本文： | 郑元仙,李永忠,刘雅婷,徐小刚,叶茂,朱秋燕.云南省蝴蝶兰上凤仙花坏死斑病毒的鉴定[J].园艺学报,2010,37(2):313-318. |
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| 作者姓名： | 郑元仙 李永忠 刘雅婷 徐小刚 叶茂 朱秋燕 |
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| 作者单位： | 1. 云南农业大学农学与生物技术学院,昆明,650201
2. 云南农业大学烟草学院,昆明,650201 |
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| 基金项目： | 国家自然科学基金项目，云南省自然科学基金面上项目，云南省教育厅自然科学基金项目，国家'973'项目 |
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| 摘 要： | 2008年对云南省主要花卉基地进行调查,获得症状表现为环斑、坏死,疑似番茄斑萎病毒属(Tospovirus)病毒的蝴蝶兰样品。应用生物测定,结合现代电镜技术、免疫试纸条检测技术初步确定为番茄斑萎病毒属的凤仙花坏死斑病毒(Impaliens necrotic spot virus,INSV),通过设计的4对引物进行RT-PCR扩增和测序,获得核壳体蛋白(Nucleocapsid protein)基因核酸长度为886nts,RNAM编码的非结构蛋白(Nonstructure protein,NSm)基因核酸长度为681nts,依赖于RNA的RNA聚合酶(RdRp)基因核酸长度为1048nts,测序结果在NCBIBLAST网络数据库中比对,同时应用生物信息软件DNAman比对,其与INSV的同源性都达90%以上,都证明分离到的病原物为凤仙花坏死斑病毒(INSV)。
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| 关 键 词： | 蝴蝶兰 蝴蝶兰环斑病 凤仙花坏死斑病毒 病毒病 |
| 收稿时间： | 2009-10-10 |
| 修稿时间： | 2010-1-4 |
Identification of Impatiens necrosis spot virus from Phalaenopsis amabilis in Yunnan |
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| ZHENG Yuan-xian,LI Yong-zhong ,LIU Ya-ting,XU Xiao-gang,YE Mao, ZHU Qiu-yan.Identification of Impatiens necrosis spot virus from Phalaenopsis amabilis in Yunnan[J].Acta Horticulturae Sinica,2010,37(2):313-318. |
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| Authors: | ZHENG Yuan-xian LI Yong-zhong LIU Ya-ting XU Xiao-gang YE Mao ZHU Qiu-yan |
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| Institution: | ZHENG Yuan-xian1,LI Yong-zhong 2,LIU Ya-ting1,XU Xiao-gang1,YE Mao1,, ZHU Qiu-yan1 (1College of Agricultural , Biotechnology,Yunnan Agricultural University,Kunming 650201,China,2College of Tobacco Science,China) |
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| Abstract: | Tospovirus,the only genus to infect plants in the Bunyaviridae family,can cause many crops and flowers to the serious disease in the tropics,the subtropics as well as the temperate zone area.This research surveyed the main flower fields of Yunnan Province in 2008.One sample with concentric rings spots and necrosis spots symptom was collected from Phalaenopsis amabilis in Kunming.By the biological techniques,electron microscope and ImmuStrip Test,it was confirmed that the plant was infected by Impatiens necr... |
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| Keywords: | Phalaenopsis amabilis impatiens ring spot disease Impatiens necrosis spot virus viral diseases |
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