| 猪瘟病毒云南毒株E2基因片段序列测定及分析 |
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| 引用本文： | 尹革芬,朱建波,姚俊,高华峰,信爱国,张念祖.猪瘟病毒云南毒株E2基因片段序列测定及分析[J].中国预防兽医学报,2001,23(3):167-169. |
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| 作者姓名： | 尹革芬 朱建波 姚俊 高华峰 信爱国 张念祖 |
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| 作者单位： | 云南农业大学动物科学技术学院, |
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| 摘 要： | 用RT－nPCR方法，选择猪瘟病毒2000年云南部分地方流行毒株E2基因主要保护性抗原决定簇编码区片段进行扩增和cDNA序列分析，并与中国标准强毒石门株进行比较。结果显示，云南地方毒株之间核苷酸及氨基酸序列同源性均大于90％；但与中国标准强毒石门株差异较大，其核苷酸及氨基酸同源性均小于80％。
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| 关 键 词： | 猪瘟病毒 E2基因 序列分析 |
| 文章编号： | 1008-0589(2001)03-0167-03 |
| 修稿时间： | 2000年9月25日 |
Sequencing Analyses of E2 Gene of Classical Swine Fever Virus Yunnan Strain |
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| YIN Gefen ,ZHU Jianbo ,YAO Jun ,GAO Huafeng ,XIN Aiguo ,ZHANG Nianzu.Sequencing Analyses of E2 Gene of Classical Swine Fever Virus Yunnan Strain[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(3):167-169. |
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| Authors: | YIN Gefen ZHU Jianbo YAO Jun GAO Huafeng XIN Aiguo ZHANG Nianzu |
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| Institution: | YIN Gefen 1,ZHU Jianbo 2,YAO Jun 2,GAO Huafeng 2,XIN Aiguo 2,ZHANG Nianzu 2 |
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| Abstract: | Using RT_nPCR method,the main epitope of E2 gene of some classical swine fever virus(CSFV) Yunnan strain was amplified,sequenced,and then compared with Shimen strain.The results showed that the nucleotide and deduced amino acid sequence homology of three Yunnan strains were higher than 90%,but there was apparentey different homology of nucleotide and amino acid sequence between Yunnan strains and Shimen strains,and the nucleotide sequence homology and the amino acid sequence homology were all lower than 80%. |
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| Keywords: | CSFV E2 gene Sequence analysizing |
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