| 马铃薯新品种桂农薯1号组培快繁研究 |
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| 引用本文： | 许娟,郑虚,韦民政,唐秀桦,闫海锋,熊军,李韦柳,覃维治.马铃薯新品种桂农薯1号组培快繁研究[J].南方农业学报,2014,0(3):383-388. |
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| 作者姓名： | 许娟 郑虚 韦民政 唐秀桦 闫海锋 熊军 李韦柳 覃维治 |
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| 作者单位： | 广西农业科学院 经济作物研究所,南宁,530007 |
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| 基金项目： | 广西科学研究与技术开发计划项目广西自然科学基金国家现代农业产业技术体系广西创新团队建设专项项目 |
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| 摘 要： | 【目的】探索马铃薯新品种桂农薯1号组培快繁技术,为实现工厂化生产脱毒种薯提供技术支撑。【方法】采用茎尖培养脱毒法,以马铃薯顶芽为外植体,按不同灭菌时间进行单因子(3%NaClO和0.1%HgCl2)和双因子(75%酒精和0.1%HgCl2)消毒处理,再剥取0.3~0.7 mm茎尖接种到分别添加了不同浓度GA3、6-BA及6-BA和NAA的诱导培养基上诱导成苗。将诱导出的苗进行单腋芽切段快繁,并接种到添加有6-BA和PP333的单因子及双因子组合MS培养基中进行无菌苗继代增殖。【结果】75%酒精+0.1%HgCl2双因子灭菌较单因子3%NaClO和0.1%HgCl2效果好,污染率降至21.67%;茎尖诱导培养中,GA3和6-BA均能诱导茎尖萌芽,诱导趋势均先增后减,而以1.5 mg/L 6-BA和0.2 mg/L NAA配合,诱导率达53.67%;增殖壮苗阶段,0.1 mg/L PP333和2.5 mg/L 6-BA配合,增殖倍数达6.59倍,且苗长势好,茎粗壮、叶色正常。【结论】桂农薯1号外植体消毒最佳灭菌处理为:75%酒精2 min+0.1%HgCl211 min;最佳茎尖诱导配方为:MS+1.5 mg/L 6-BA+0.2 mg/L NAA;适合继代增殖壮苗培养基为:MS+2.5 mg/L 6-BA+0.1 mg/L PP333。
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| 关 键 词： | 桂农薯1号 组织培养 茎尖 消毒 芽诱导 增殖 |
Tissue culture and rapid propagation of a new potato variety Guinongshu 1 |
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| XU Juan,ZHENG Xu,WEI Min-zheng,TANG Xiu-hua,YAN Hai-feng,XIONG Jun,LI Wei-liu,QIN Wei-zhi.Tissue culture and rapid propagation of a new potato variety Guinongshu 1[J].Journal of Southern Agriculture,2014,0(3):383-388. |
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| Authors: | XU Juan ZHENG Xu WEI Min-zheng TANG Xiu-hua YAN Hai-feng XIONG Jun LI Wei-liu QIN Wei-zhi |
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| Abstract: | Objective]The tissue culture and rapid propagation of a new potato variety Guinongshu 1 were studied to pro-vide technical support for industrializing production of virus-free potato seeds.Method]The stem tip detoxification method was adopted and stem tip was used as explants. The single factor (3% NaClO and 0.1% HgCl2) and two factors (75% alcohol and 0.1%HgCl2) disinfection treatments were conducted at different sterilization times. Stem tip with the size of 0.3-0.7 mm were induced into seedlings in induction medium with different concentrations of GA3, 6-BA and combinations of 6-BA and NAA. The induced single lateral bud was cut and inoculated in MS culture medium of single factor and two factors combina-tion added with 6-BA and PP33 to proliferate. Result]The sterilization effect of two factors of 75%alcohol+0.1%HgCl2 was better than single factor of 3%NaClO or 0.1%HgCl2, and the pollution rate declined to 21.67%. Both GA3 and 6-BA could in-duce germination of stem tip with an induced trend of increase-decrease. The induction rate was as high as 53.67%for combi-nation of 1.5 mg/L 6-BA and 0.2 mg/L NAA. At multiplication stage, the multiplication rate of combination treatment adding 0.1 mg/L PP333 and 2.5 mg/L 6-BA was as high as 6.59 times and the seedlings grew better with strong stems and leaves, and normal color. Conclusion]For Guinongshu 1 explants, the best sterilization treatment was 75%alcohol 2 min+0.1%HgCl2 11 min and the best stem tip induction formula was MS+1.5 mg/L 6-BA+0.2 mg/L NAA. The best multiplication medium was MS+2.5 mg/L 6-BA+0.1 mg/L PP333. |
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