| 羧基化壳寡糖络合碘（CCOS-I）对水稻纹枯病菌的抑制作用及机理初讨 |
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| 引用本文： | 胡雪芳,田志清,梁亮,朱祥民,姜昊业,王士奎,裴海生.羧基化壳寡糖络合碘（CCOS-I）对水稻纹枯病菌的抑制作用及机理初讨[J].热带作物学报,2018,39(8):1590-1595. |
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| 作者姓名： | 胡雪芳 田志清 梁亮 朱祥民 姜昊业 王士奎 裴海生 |
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| 作者单位： | 1农业农村部规划设计研究院农产品加工工程研究所；
2农业农村部农产品产后处理重点实验室；
3上海贯发海洋生物科技有限公司 |
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| 摘 要： | 为明确羧基化壳寡糖络合碘（CCOS-I）对水稻纹枯病的防治作用，以 20%井冈霉素可湿性粉剂为对照药剂，测定 CCOS-I 对水稻纹枯病菌的室内抑菌效果和田间防效，以及对超氧化物歧化酶（superoxidedismutase，SOD）、叶片过氧化物酶（peroridase，POD）、多酚氧化酶（polyphenol oxidase，PPO）、苯丙氨酸解氨酶（phenylalanine ammonia-lyase，PAL）和 β-1,3-葡聚糖酶（β-1,3-glucanase）等相关防御酶活性的影响。结果表明：羧基化壳寡糖络合碘对该病菌抑制效果明显，经室内毒力测定，其 EC50 值 12.22 mg/L，明显高于对照药剂 20%井冈霉素粉剂。田间药效试验结果表明，在水稻第 3 次用药后 14 d，100 g/hm2 的羧基化壳寡糖络合碘防效达到 80.66%，与井冈霉素有效剂量 150 g/hm2 的效果相当，优于同剂量井冈霉素处理。CCOS-I 在试验剂量范围内对水稻纹枯病有很好的防治作用，且对水稻生长无任何药害现在发生，可以诱导水稻相关防御酶活性提高，适用于水稻纹枯病的防治，具有一定的开发推广价值。
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| 关 键 词： | 水稻纹枯病菌（Rhizoctoniasolani） 羧基化壳寡糖络合碘（CCOS-I） 抑菌作用 防御酶 防治效果 |
The Effect of Chitosan Oligosaccharide Complex Iodine (CCOS-I) on the Inhibition of Rice Rhizoctonia solani and the Effect of Defense Enzyme Activity Inhibitory |
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| HU Xuefang,TIAN Zhiqing,LIANG Liang,ZHU Xiangmin,JIANG Haoye,WANG Shikui,PEI Haisheng.The Effect of Chitosan Oligosaccharide Complex Iodine (CCOS-I) on the Inhibition of Rice Rhizoctonia solani and the Effect of Defense Enzyme Activity Inhibitory[J].Chinese Journal of Tropical Crops,2018,39(8):1590-1595. |
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| Authors: | HU Xuefang TIAN Zhiqing LIANG Liang ZHU Xiangmin JIANG Haoye WANG Shikui PEI Haisheng |
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| Institution: | 1Institute of Agricultural Products Processing Engineering Research, Chinese Academy of Agricultural Engineering Planning & Design, Beijing 100125, China;
2Key Laboratory of Agro-Products Postharvest Handling, Ministry of Agriculture and Rural Affairs,；
3Shanghai Guanfa Marine Biological Technology |
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| Abstract: | In order to clarify the preventive and therapeutic effects on rice sheath blight, 20% validamycin wettable
powder as the control, the antibacterial effect of carboxylated chitosan oligosaccharide iodine complex (CCOS-I) on R.solani in a greenhouse and its efficacy in the paddy field were assayed. And the induced activity of CCOS-I on the peroxidase (POD), superoxide dismutase (SOD), polyphenol oxidase (PPO), phenylalnaine ammonia-lyase (PAL) and beta-1,3-glucanase (beta-1,3-glucanase) were studied. CCOS-I had a significant inhibitory effect on R. solani, and its EC50 value was 12.22 mg/L, which was obviously higher than that of the control in the field trial test, The control effect of CCOS-I at the effective dose of 100 g/hm2 was 80.66%，which was equivalent to the valid dose of validamycin 150 g/hm2 and better than the same dose of validamycin，after the third spraying 14 d. Compared with the spraying control agent, CCOS-I not only had good control effect on rice sheath blight, but also had a certain promoting effect on rice growth. CCOS-I induced rice resistance against R. solani which related to the increased activities of defense enzymes caused by CCOS-I. CCOS-I was safe for rice and had a certain value of development and promotion. |
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| Keywords: | rice sheath blight (Rhizoctonia solani) carboxyl chitosan oligosaccharide complex iodine (CCOS-I) antimicrobial activity defense |
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