| 混栽对主栽作物茄子根际土壤微生物生态学特性的影响 |
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| 引用本文： | 姚华开,张传进,刘岳飞,吴人敏,杨尚东.混栽对主栽作物茄子根际土壤微生物生态学特性的影响[J].热带作物学报,2018,39(6):1067-1074. |
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| 作者姓名： | 姚华开 张传进 刘岳飞 吴人敏 杨尚东 |
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| 作者单位： | 1广西大学农学院；
2广西农业科学院
3广西甘蔗遗传改良重点实验室 |
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| 摘 要： | 探索构建微生物多样性丰富根际微环境的方法，为生态防控连作障碍提供理论依据与技术支撑。选取茄子为主栽作物，苋菜、薄荷、芥菜、韭菜、菜豆等匍匐生长或矮生的蔬菜种类为混栽组合，采用传统分析方法和现代高通量测序技术，分析混栽对主栽茄子根际土壤肥力及细菌多样性的影响。结果表明：混栽组合茄子根际土壤中可培养微生物数量(细菌、真菌、放线菌)，涉及碳、氮、磷循环相关酶的活性以及微生物生物量(碳、氮、磷)等指示土壤肥力与健康状况的生物学指标，均优于单一种植的对照；另一方面，基于高通量测序技术分析发现，混栽组合茄子根际土壤中细菌的多样性指数(H)、丰度(S)和均匀度指数(Eh)均显著高于对照。上述茄子混栽组合种植均能有效地提高主栽作物茄子根际土壤肥力和细菌多样性。其中，茄子-菜豆和茄子-韭菜组合对提高茄子根际土壤肥力和细菌多样性的效果尤为显著。
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| 关 键 词： | 茄子 套种 高通量测序 细菌多样性 |
Effects of Intercropping on Soil Characteristics of Microbial Ecology in Rhizosphere of Eggplant |
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| YAO Huakai,ZHANG Chuanjin,LIU Yuefei,WU Renmin,YANG Shangdong.Effects of Intercropping on Soil Characteristics of Microbial Ecology in Rhizosphere of Eggplant[J].Chinese Journal of Tropical Crops,2018,39(6):1067-1074. |
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| Authors: | YAO Huakai ZHANG Chuanjin LIU Yuefei WU Renmin YANG Shangdong |
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| Institution: | 1Agricultural College, Guangxi University;
2Guangxi Key Laboratory of Sugarcane Genetic Improvement
3Guangxi Academy of Agricultural Sciences |
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| Abstract: | To reduce the harm of continuous cropping obstacle, a bio-control method via building high microbial diversity in the rhizosphere of crops was explored. Eggplant (Solanaceae melongena L.) was selected as a main crop and amaranth (Amaranthus tricolor Linn.), mint (Mentha haplocalyx Briq.), mustard(Brassica Juncea Coss.), leek(Allium tuberosum Rottler ex Prengel.)and kidney beans(Phaseolus vularis L.), which belonging to low-growing or creeping plants, were selected as the associated plants, respectively. Based on modern and traditional analyzed techniques, the effect of intercropping on soil fertility and bacterial diversity in the rhizosphere of eggplant were analyzed. The results showed that bio-indicators of soil fertility, such as the amount of cultivable microorganisms (bacteria, fungi, actinomycetes), enzyme activities and microbial biomass, which related to carbon, nitrogen and phosphorus cycles in soils of intercropping treatments, were all higher than those of the control. In addition, the indexes of soil bacterial diversity (H), abundance(S) and richness in the rhizosphere of eggplant also could be improved by intercropping treatments. It indicates that soil fertility and bacterial diversity in the rhizosphereof eggplants could be enhanced via intercropping methods, of which, kidney bean and leek are the most effective associated plants of eggplant. |
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| Keywords: | eggplant intercropping high throughput sequencing bacterial diversity |
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