| 牛樟 EST-SSR 标记的开发及遗传多态性分析 |
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| 引用本文： | 郭莺,孟红岩,林文珍,林志楷,刘黎卿.牛樟 EST-SSR 标记的开发及遗传多态性分析[J].热带作物学报,2018,39(8):1561-1569. |
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| 作者姓名： | 郭莺 孟红岩 林文珍 林志楷 刘黎卿 |
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| 作者单位： | 福建省亚热带植物研究所 |
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| 摘 要： | 本研究通过测序牛樟叶片 cDNA 获得 17 046 条 Uni-EST，从中检测出 1 316 个 SSR 位点，平均每 25.7 kb EST 含 1 个 SSR 位点。功能注释发现 1 242 条 SSR-EST 中有 690 条可被划分为 3 大功能类、18 个功能亚类。牛樟 EST-SSR 二核苷酸重复类型出现频率最高，三核苷酸次之。184 类重复基序中 AG/CT 出现频率最高（32.4%），其次为 AAG/CTT（9.3%）。从 1 242 条 SSR-EST 设计 SSR 引物 537 对，随机选取 150 对检测牛樟的多态性及在香樟、大叶樟和沉水樟中的可转移性。结果表明，有 44 对引物在牛樟中得到特异性扩增，其中 17 对具有多态性，检测出的等位基因数 2~7 个，平均 3.02 个。牛樟 SSR 标记在香樟、沉水樟和大叶樟的可转移率分别为 97.7%、97.7%和 95.3%。聚类分析表明，在遗传相似系数为 0.76 处，14 个樟属植物能被划分为 5 类，聚类结果与植物学分类结果基本吻合。
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| 关 键 词： | 牛樟 EST-SSR 标记开发 遗传多态性 |
Developing EST-SSR Markers in Cinnamomum kanehirae and Analyzing the Genetic Polymorphism |
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| GUO Ying,MENG Hongyan,LIN Wenzhen,LIN Zhikai,LIU Liqing.Developing EST-SSR Markers in Cinnamomum kanehirae and Analyzing the Genetic Polymorphism[J].Chinese Journal of Tropical Crops,2018,39(8):1561-1569. |
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| Authors: | GUO Ying MENG Hongyan LIN Wenzhen LIN Zhikai LIU Liqing |
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| Institution: | Fujian Institute of Subtropical Botany |
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| Abstract: | 17 046 Uni-ESTs were generated by sequencing cDNA of Cinnamomum kanehirae, of which 1 316 SSR loci were identified with 1 SSR on every 25.7 kb EST. Analysis of GO (gene ontology) showed that 690 SSR-ESTs could be divided into three major functional categories and 18 functional sub-categories. Di-nucleotide repeat was the most abundant repeat type followed by tri-nucleotide repeat in EST of C. kanehirae. In 184 motif types, AG/CT (32.4%) was the most abundant followed by AAG/CTT (9.3%). Based on the 1 242 SSR-EST, a total of 537 primer pairs were successfully designed. 150 primer pairs were randomly selected to detect the polymorphism in C. kanehirae and test transferability in C. camphora, C. micranthum and C. parthenoxylon. The results showed that 44 primer pairs were specifically amplified in C. kanehirae, of which 17 pairs were polymorphic and the number of alleles detected was 2–7, with an average of 3.02. The transfer rates of C. kanehirae SSR marker in C. camphora, C. micranthum and C. parthenoxylon were 97.7%, 97.7% and 95.3%, respectively. Cluster analysis showed that at the genetic similarity coefficient 0.76, 14 Cinnamomum plant samples could be divided into five groups. The clustering result was in good agreement with the result of botany classification. |
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| Keywords: | Cinnamomum kanehirae EST-SSR marker development genetic polymorphism |
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