| 真菌诱导子对龙眼胚性愈伤组织中类黄酮、类胡萝卜素和单宁含量的影响 |
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| 引用本文： | 刘生财,段俊朋,徐小萍,潘君飞,程春振,林玉玲,赖钟雄.真菌诱导子对龙眼胚性愈伤组织中类黄酮、类胡萝卜素和单宁含量的影响[J].热带作物学报,2018,39(9):1778-1785. |
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| 作者姓名： | 刘生财 段俊朋 徐小萍 潘君飞 程春振 林玉玲 赖钟雄 |
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| 作者单位： | 福建农林大学园艺植物生物工程研究所 |
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| 摘 要： | 为探讨真菌诱导子与龙眼胚性愈伤组织中类黄酮、类胡萝卜素和单宁含量的相关性，以龙眼胚性愈伤组织细胞为材料，研究真菌诱导子种类、浓度及处理天数对龙眼愈伤组织细胞中类黄酮、类胡萝卜素和单宁含量的影响。结果表明：在固体培养基中加入 100 mg/L 拟茎点霉菌诱导子培养 35 d 时，最有利于类黄酮含量的积累，含量高达 8.58 mg/g；拟茎点霉菌诱导子浓度为 50 mg/L 时培养 35 d，最有利于类胡萝卜素含量的积累，其含量为36.83 μg/g；胶孢镰刀菌诱导子浓度为 50 mg/L 时的培养基中培养 35 d，最有利于单宁含量的积累，其含量为10.50 mg/g。液体悬浮培养条件下，胶孢镰刀菌诱导子浓度为 400 mg/L 的培养基中培养 7 d，最有利于类黄酮含量的积累，其含量为 6.29 mg/g；尖孢镰刀菌诱导子浓度为 200 mg/L 的培养基中培养 7 d，最有利于类胡萝卜素含量的积累，其含量为 49.21 μg/g；胶孢镰刀菌诱导子浓度为 400 mg/L 的培养基中培养 7 d，最有利于单宁含量的积累，其含量为 13.15 mg/g。本研究为将来龙眼胚性愈伤组织细胞工厂化生产类黄酮、类胡萝卜素和单宁等次生代谢物质奠定理论基础并提供技术指导。
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| 关 键 词： | 龙眼 胚性愈伤组织 真菌诱导子 类黄酮 类胡萝卜素 单宁 |
Effects of Fungal Elicitors on the Content of Flavonoids,Carotenoids and Alkaloids in Embryogenic Calli of Dimocarpus longan Lour |
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| LIU Shengcai,DUAN Junpeng,XU Xiaoping,PAN Junfei,CHENG Chunzhen,LIN Yuling,LAI Zhongxiong.Effects of Fungal Elicitors on the Content of Flavonoids,Carotenoids and Alkaloids in Embryogenic Calli of Dimocarpus longan Lour[J].Chinese Journal of Tropical Crops,2018,39(9):1778-1785. |
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| Authors: | LIU Shengcai DUAN Junpeng XU Xiaoping PAN Junfei CHENG Chunzhen LIN Yuling LAI Zhongxiong |
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| Institution: | Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University |
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| Abstract: | To study the correlation between fungi elicitors and flavonoids, carotenoids and tannins in the embryogenic
calli of longan, the embryogenic calli were used as the materials to investigate the effects of different kinds of fungi elicitors for the contents of flavonoids, carotenoids and tannin in callus cells. The results showed that under the condition of solid culture, the flavonoid content was the highest (8.58 mg/g) under the treatment of 100 mg/L Phomopsis longanae Chi for 35 d, and the carotenoid content was the highest (36.83 μg/g) under 50 mg/L P. longanae Chi treatment. For tannin, however, the content was the highest (10.50 mg/g) under Fusarium subglutinans Wollenw. et Reinkin treatment for 35d. Under liquid culture, the flavonoid content was the highest (6.29 mg/g) under the treatment of 400 mg/L F. subglutinans Wollenw. et Reinkin treatment for 7 d, the carotenoid content was the highest (49.21 μg/g) under the treatment of 200 mg/L F. Oxysporum for 7 d, and the tannin content was the highest (13.15 mg/g) under 400 mg/L F. subglutinans Wollenw. et Reinkin treatment for 7 d. The above results could provide a scientific basis and technical support for studying the metabolic mechanism of flavonoids, carotenoids and alkaloids． |
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| Keywords: | Dimocarpus longan Lour embryogenic calli fungal elicitors flavonoids carotenoids tannins |
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