| 新鲜椰衣纤维表面半纤维素含量的测定及去除 |
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| 引用本文： | 王政,黄广民,赵斌,李山丹,胡旦.新鲜椰衣纤维表面半纤维素含量的测定及去除[J].热带作物学报,2018,39(1):156-161. |
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| 作者姓名： | 王政 黄广民 赵斌 李山丹 胡旦 |
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| 作者单位： | 海南大学食品学院 |
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| 摘 要： | 新鲜椰衣纤维表面粘附有一定量结构疏松的椰糠,椰糠的主要成分为半纤维素和木聚糖类,既不利于椰衣纤维的改性,也不利于用做固定化酶的载体材料,必须将其除去。采用稀硫酸加热水解,使新鲜椰衣纤维表面粘附的半纤维素转化为可溶的木糖和低聚木糖,以木糖为标准,3,5-二硝基水杨酸(DNS)为显色剂,采用分光光度法间接测定新鲜椰衣纤维中半纤维的含量,以考察椰衣纤维表面半纤维去除的效果。结果表明,当硫酸溶液浓度1.3 mol/L、水解温度102℃、水解时间2 h,固液比为1∶50时,半纤维素的去除效果最佳,新鲜椰衣纤维表面粘附的半纤维素量为14.58%,通过红外光谱和扫描电镜等可观察椰衣纤维表面的变化,重现性试验及标准回收试验证明该方法效果良好。
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| 关 键 词： | 椰衣纤维 硫酸加热水解 分光光度法 半纤维素 |
Determination and Removal of Hemicellulose Content on the Surface of Coir Fiber |
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| WANG Zheng,HUANG Guangmin,ZHAO Bin,LI Shandan,HU Dan.Determination and Removal of Hemicellulose Content on the Surface of Coir Fiber[J].Chinese Journal of Tropical Crops,2018,39(1):156-161. |
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| Authors: | WANG Zheng HUANG Guangmin ZHAO Bin LI Shandan HU Dan |
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| Institution: | College of Food Science and Technology, Hainan University, Haikou, Hainan 570228, China |
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| Abstract: | Fresh coconut fiber has a certain amount of loose coconut bran on its surface. The main components of coco coir are hemicellulose and pentosan, not conducive to modifying coir fiber, must be removed. Dilute sulphuric acid was used to remove the hemicellulose bran on the surface of fresh coconut fiber, and the content of hemicellulose was determined by spectrophotometry. The effect of hemicellulose removal on coir fiber was investigated. The results showed the highest removal of hemicellulose could be achieved when hydrolyzed for 2 h in 1.3 mol/L sulfuric acid with a solid-liquid ratio 1 ∶ 5 under 102 ℃. Under the above conditions the hemicellulose adhesion to the surface of semi fresh coconut fiber was 14.58%. Reproducibility test and standard recovery test confirmed the method was effective. The change of the surface of coconut fiber was observed under infrared scanning electron microscope. |
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| Keywords: | fresh coconut crude fiber atmospheric sulfuric acid hydrolysis method spectrophotometry hemicellulose |
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