| 贵州惠水县茶轮斑病病原菌的鉴定 |
| |
| 引用本文： | 李冬雪,赵晓珍,王勇,练珊珊,任亚峰,陈卓.贵州惠水县茶轮斑病病原菌的鉴定[J].热带作物学报,2018,39(9):1827-1833. |
| |
| 作者姓名： | 李冬雪 赵晓珍 王勇 练珊珊 任亚峰 陈卓 |
| |
| 作者单位： | 1贵州大学绿色农药与农业生物工程教育部重点实验室；
2贵州省果树科学研究所；
3贵州大学农学院；
4贵州大学茶学院 |
| |
| 摘 要： | 贵州省惠水县茶区常发生茶轮斑病，对茶叶品质和产量影响较大。为确定该病害的病原菌，本文对病害叶片的病原菌进行分离、纯化和培养，通过柯赫氏法则进行病原菌针刺法和剪切法的致病性测定。并依据病原菌核糖体内转录间隔区、β-微管蛋白、延伸因子-1α 进行多基因系统发育分析。基于病原菌多基因系统发育和形态学结果，将病原菌确定为茶假拟盘多毛孢（Pseudopestalotiopsis camelliae-sinensis）。
|
| 关 键 词： | 茶树 茶轮斑病 假拟盘多毛孢属 形态特征 致病性分析 系统发育分析 |
Identification of the Pathogen Causing Tea Gray Blight Disease in Huishui County,Guizhou Province |
| |
| LI Dongxue,ZHAO Xiaozhen,WANG Yong,LIAN Shanshan,REN Yafeng,CHEN Zhuo.Identification of the Pathogen Causing Tea Gray Blight Disease in Huishui County,Guizhou Province[J].Chinese Journal of Tropical Crops,2018,39(9):1827-1833. |
| |
| Authors: | LI Dongxue ZHAO Xiaozhen WANG Yong LIAN Shanshan REN Yafeng CHEN Zhuo |
| |
| Institution: | 1Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, Guizhou 550025, China;
2. Guizhou Institute of Fruit Tree Science, Guiyang, Guizhou 550006, China;
3. Agricultural College, Guizhou University, Guiyang, Guizhou 550025, China;
4. Tea College, Guizhou University, Guiyang, Guizhou 5 |
| |
| Abstract: | Tea gray blight disease always takes place in the tea area of Huishui County, Guizhou Province, which severely affects the quality and yield of tea leaves. To determine the pathogen of the disease, we isolated, purified and cultivated the pathogenic fungus from the diseased tea leaves, then determined its pathogenicity following Koch’s rule with the methods of needle punch and cut. Phylogenetic analyses against multi-locus sequences, viz. rDNA-ITS, β-tublinand EF-1α were conducted. Based on the morphology and phylogenetic analyses, the pathogen was identified as Pseudopestalotiopsis camelliae-sinensis. |
| |
| Keywords: | Camellia sinensis tea gray blight disease Pseudopestalotiopsis morphological characteristics pathogenicity analysis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
