| 应用流式细胞术研究犬瘟热病毒感染细胞的凋亡 |
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| 引用本文： | 郭爱珍,陆承平,张汇东.应用流式细胞术研究犬瘟热病毒感染细胞的凋亡[J].中国兽医学报,2000,20(5). |
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| 作者姓名： | 郭爱珍 陆承平 张汇东 |
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| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室!江苏南京210095(郭爱珍，陆承平)，公安部南京警犬研究所(张汇东) |
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| 摘 要： | 用犬瘟热病毒 ( CDV) 2种不同毒株感染非洲绿猴肾细胞 ( Vero) ,用流式细胞术分析了病毒诱导的感染细胞的凋亡。结果发现 ,2种毒株感染引起的细胞病变类型不同 ,分为圆缩型与合胞体型 ;2种毒株均可诱导感染细胞凋亡 ,但毒株间的凋亡细胞比例存在差异 ,即产生合胞体型细胞病变的毒株凋亡出现较晚 ,凋亡细胞比例显著低于产生圆缩型细胞病变的毒株 ( P<0 .0 1 )。结果提示 ,不同毒株导致细胞死亡的机制可能不同
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| 关 键 词： | 犬瘟热病毒 细胞凋亡 流式细胞术 |
Study on the Apoptosis Induced by CDV Using the Flow Cytometry |
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| GUO Ai zhen ,LU Cheng ping ,ZHANG Hui dong.Study on the Apoptosis Induced by CDV Using the Flow Cytometry[J].Chinese Journal of Veterinary Science,2000,20(5). |
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| Authors: | GUO Ai zhen LU Cheng ping ZHANG Hui dong |
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| Institution: | GUO Ai zhen 1,LU Cheng ping 1,ZHANG Hui dong 2 |
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| Abstract: | Two canine distemper virus (CDV) strains infected Vero cells and flow cytometry were used to analyse the apoptosis induced by CDV.The results showed that both CDV strains could induce apoptosis of infected cells.However,there was significant difference in the percentage of apoptotic cells between the two strains (P<0.01).The strain resulted in the CPE type of cell rounding induced much higher ratio of apoptotic cells than the strain resulted in the CPE type of syncytium.It suggested that the mechanism of cell death may be different after infection of various CDV strains. |
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| Keywords: | canine distemper virus(CDV) apoptosis flow cytometry |
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