CaCl_2对盐胁迫下大豆幼苗渗透调节物质含量的影响 |
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引用本文: | 杨艳波,刘若岑,崔欣,张元,库孜拉·俄斯别克,武燕.CaCl_2对盐胁迫下大豆幼苗渗透调节物质含量的影响[J].安徽农业科学,2016(25). |
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作者姓名: | 杨艳波 刘若岑 崔欣 张元 库孜拉·俄斯别克 武燕 |
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作者单位: | 大庆师范学院,黑龙江大庆,163712 |
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摘 要: | 目的]为土壤盐渍化地区的大豆种植提供合理化建议,提高大豆产量。方法]采用盆栽法培养大豆,分析10、15和20 mmol/L外源CaCl_2对150 mmol/L Na Cl胁迫下大豆幼苗渗透调节物质含量的影响。结果]三叶期大豆幼苗施加150 mmol/L NaCl胁迫后,可溶性糖和可溶性蛋白的含量与空白对照组相比均显著升高(P0.05),而脯氨酸含量显著下降(P0.05);喷施10 mmol/L CaCl_2处理组与盐对照组相比,可溶性糖和脯氨酸含量显著升高(P0.05),可溶性蛋白含量显著下降(P0.05);喷施15 mmol/L CaCl_2处理组与盐对照组相比,3种渗透调节物质含量均升高,其中可溶性糖、脯氨酸含量升高显著(P0.05),可溶性蛋白含量升高不显著(P0.05);喷施20 mmol/L CaCl_2处理组与盐对照组相比,可溶性糖、可溶性蛋白含量降低,但未达到显著水平(P0.05),而脯氨酸含量显著升高(P0.05)。结论]综合分析外源CaCl_2对盐胁迫下大豆幼苗渗透调节物质含量的影响表明,15 mmol/L CaCl_2的缓解效果最好。
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关 键 词: | 大豆 盐胁迫 CaCl2 渗透调节物质 |
Effects of CaCl2 on the Content of Osmotic Adjustment Substances in Soybean Seedlings under Salt Stress |
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Abstract: | Objective ] The aim was to provide rationalization proposals for soybean planting in soil salinization area , and increase soybean yield.Method] By using pot cultured soybean, effects of 10,15 and 20 mmol/L exogenous CaCl2on osmotic adjustment substances in soybean seedlings under NaCl 150mmol/L stress were studied.Result] Applying 150 mmol/L NaCl on soybean seedling in three leaf stage, the content of soluble sugar and soluble protein were significantly higher than (P<0.05) that in the blank control group, and the proline content was de-creased significantly (P<0.05); spraying 10 mmol/L CaCl2 treatment group was compared with the salt control group, the contents of proline and soluble sugar were increased significantly (P<0.05), soluble protein was significantly decreased (P<0.05); spraying 15 mmol/L CaCl2 treatment group was compared with salt control group, the content of osmotic substances were increased, the soluble sugar, proline content were increased significantly (P<0.05), soluble protein was not significant(P>0.05); spraying 20 mmol/L CaCl2 treatment group was compared with the salt control group, soluble sugar, soluble protein content were decreased, but did not reach significant level (P>0.05), and the proline content was increased significantly (P<0.05).Conclusion]The effect of exogenous CaCl2 on the content of osmotic adjustment substances of soybean seedlings under salt stress showed that 15 mmol/L CaCl2 had the best effect. |
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Keywords: | Soybean Salt stress Calcium chloride Osmotic adjustment substance |
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