| 两步培养法测定厚垣孢普可尼亚菌产孢的最佳条件 |
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| 引用本文： | 石妍,肖顺,刘国坤,张绍升.两步培养法测定厚垣孢普可尼亚菌产孢的最佳条件[J].安徽农业科学,2016,44(26):16-19. |
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| 作者姓名： | 石妍 肖顺 刘国坤 张绍升 |
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| 作者单位： | 福建农林大学植物保护学院,福建福州,350002;福建农林大学植物保护学院,福建福州,350002;福建农林大学植物保护学院,福建福州,350002;福建农林大学植物保护学院,福建福州,350002 |
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| 基金项目： | 中国烟草总公司福建公司科技项目(闽烟2012[041]号)。 |
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| 摘 要： | 目的]探讨厚垣孢普可尼亚菌产厚垣孢子的条件。方法]采用两步培养法测定碳源、氮源、碳氮摩尔比、pH等不同营养环境条件对厚垣孢普可尼亚菌PC021120-152菌株产厚垣孢子的影响。结果]PC021120-152菌株的最佳产厚垣孢子条件为:初始碳源浓度为3.00 g/L,碳氮摩尔比为2.5∶1,最佳碳氮源组合为蔗糖/NaNO_3,培养基最佳pH为8.0。PC021120-152菌株的最佳产分生孢子条件为:初始碳源浓度为3.00 g/L,碳氮摩尔比为2.5∶1,最佳碳氮源组合为D-果糖/NaNO_3,最佳pH为5.0。结论]该研究结果可为厚垣孢普可尼亚菌生防制剂的生产调控提供依据。
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| 关 键 词： | 厚垣孢普可尼亚菌 产孢 营养条件 |
Optimum Conditions for Sporulation of Pochonia chlamydosporia in Two-stage Cultivation |
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| SHI Yan,XIAO Shun,LIU Guo-kun,ZHANG Shao-sheng.Optimum Conditions for Sporulation of Pochonia chlamydosporia in Two-stage Cultivation[J].Journal of Anhui Agricultural Sciences,2016,44(26):16-19. |
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| Authors: | SHI Yan XIAO Shun LIU Guo-kun ZHANG Shao-sheng |
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| Abstract: | Objective] To discuss the nutritional conditions for sporulation of Pochonia chlamydosporia.Method] A two-stage cultivation meth-od was used to determine the effects of various nutritional and environmental conditions ( carbon sources,nitrogen sources,carbon-nitrogen molar ratio,and pH) on the sporulation of P.chlamydosporia PC021120-152.Result] Chlamydospore production of PC021120-152 strains was the high-est when the initial carbon concentration was 3.00 g/L;the carbon-nitrogen molar ratio was 2.5∶1;sucrose/NaNO3 was as the carbon/nitrogen source,and the pH was 8.Conidiophore production of PC021120-152 strains was the highest when the initial carbon concentration was 3.00 g/L;the carbon-nitrogen molar ratio was 2.5∶1;D-fructose/NaNO3 was as the carbon/nitrogen source,and the pH was 5.0.Conclusion] The re-search provides references for industrialization of biocontrol agents of P.chlamydosporia. |
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| Keywords: | Pochonia chlamydosporia Sporulation Nutritional conditions |
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