| 猴头菌子实体与菌丝体粗多糖理化性质及抗胃粘膜细胞氧化活性比较 |
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| 引用本文： | 肖旭朗,薛德冬,张薇,张洪贺,高阳,徐多多,王明星.猴头菌子实体与菌丝体粗多糖理化性质及抗胃粘膜细胞氧化活性比较[J].安徽农业科学,2016,44(24). |
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| 作者姓名： | 肖旭朗 薛德冬 张薇 张洪贺 高阳 徐多多 王明星 |
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| 作者单位： | 长春中医药大学药学院,吉林长春,130117;山东省东营市胜利医院药剂科,山东东营,257000;长春银诺克药业有限公司,吉林长春,130000 |
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| 基金项目： | 国家自然科学基金项目(30873370)。 |
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| 摘 要： | 目的]比较猴头菌子实体、菌丝体粗提物理化性质,以及2组粗多糖对胃粘膜上皮细胞氧化模型保护能力的差异。方法]采用水提醇沉法提取粗多糖,用苯酚-浓硫酸法测定葡萄糖含量、间羟基联苯法测糖醛酸含量、BCA法测量蛋白质含量。每组分别加入一定浓度的含粗多糖的培养基,分别培养12、24、48 h后加入含过氧化氢培养基。用MTT法测细胞活度。结果]猴头菌菌丝体、子实体粗多糖中葡萄糖含量分别为49.5%、44.5%,糖醛酸含量分别为22.01%、29.23%,蛋白质含量分别为27.69%、23.54%。菌丝体组12、24、48h抑制率分别为75.1%、68.1%、16.9%;子实体组分别为70.2%、61.8%、16.7%。结论]猴头菌菌丝体与子实体粗多糖中糖醛酸、葡萄糖、蛋白质含量相近。子实体粗多糖比菌丝体粗多糖抗氧化活性短时间内略强,长时间无差异。
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| 关 键 词： | 猴头菌菌丝体 猴头菌子实体 理化性质 胃粘膜细胞 抗氧化 |
Comparison of Physicochemical Propetr ies between Hericium Erinaceus Mycelium and Fruit Body Crude Polysaccharides and Their Anti-oxid ation Effects of Gastric Mucosal Cel |
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| Abstract: | Objective] To compare the physicochemical properties of crude polysaccharides compare of Hercium erinaceus mycelium and fruit body, and to compare the differences of the effects of two crude extracts on oxidation protection ability of gastric mucosa epithelial cell. Meth-od] Crude crude polysaccharides were extracted by water-extraction and alcohol-precipitation method.Glucose content was detected by phenol-sulphuric acid method; uronic acid content was detected by m-hydroxydiphenyl method; and protein content was detected by BCA method. Culture medium containing certain content of polysaccharides compare was added into each group.After cultivated for 12, 24 and 48 h, cul-ture medium containing hydrogen peroxide was added.Viability of cells was detected by MTT. Result] Glucose contents in mycelium and fruit body were 49.5% and 44.5%, respectively.The uronic acid contents were 22.01% and 29.23%; protein contents were 27.69% and 23.54%.The inhibitory rates of mycelium group at 12, 24 and 48 h were 75.1%, 68.1% and16 .9%, respectively;those of fruit body were 70.2%, 61.8% and 16.7%, respectively.Conclusion]In the crude crude polysaccharides of mycelium and fruit body of Hecr ium erina-ceus, contents of uronic acid, glucose and protein are close.Crude polysaccharides of fruit body have stronger antioxidant activity than extracts of mycelium in short term, but there were no differences in long term. |
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| Keywords: | Heric um Erinaceus mycelium Hercium erinaceus fruit body Physicochemical properties Gastric mucosal cell Antioxidant |
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