| 东北扁核木组培快繁研究 |
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| 引用本文： | 梁文卫,关莹,周双,王明洁,杜汉军.东北扁核木组培快繁研究[J].安徽农业科学,2016,44(29). |
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| 作者姓名： | 梁文卫 关莹 周双 王明洁 杜汉军 |
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| 作者单位： | 黑龙江省农业科学院浆果研究所,黑龙江绥棱,152204;黑龙江省农业科学院浆果研究所,黑龙江绥棱,152204;黑龙江省农业科学院浆果研究所,黑龙江绥棱,152204;黑龙江省农业科学院浆果研究所,黑龙江绥棱,152204;黑龙江省农业科学院浆果研究所,黑龙江绥棱,152204 |
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| 摘 要： | 目的]筛选东北扁核木组织培养最适宜的初代、继代和生根培养基。方法]以东北扁核木的单芽茎段为外植体,采用MS或1/2MS为基本培养基,添加不同浓度的6-BA、IBA、NAA和GA3,探讨不同培养基对东北扁核木芽诱导、增殖和生根效果的影响。结果]IBA浓度对东北扁核木初代培养的启动率影响显著,6-BA浓度和GA3浓度的影响不显著,因素主次顺序依次为IBA浓度、GA3浓度、6-BA浓度;6-BA浓度对其继代培养的增殖系数影响显著,IBA浓度和NAA浓度的影响不显著,因素主次顺序依次为6-BA浓度、IBA浓度、NAA浓度;IBA浓度对其生根培养的生根指数影响显著,NAA浓度的影响不显著,因素主次顺序依次为IBA浓度、NAA浓度。结论]东北扁核木组织培养最适宜的初代、继代和生根培养基分别为MS+0.5 mg/L 6-BA+0.2 mg/L IBA、MS+1.0 mg/L 6-BA+0.1 mg/L IBA和1/2 MS+2.0 mg/L IBA+0.1 mg/L NAA。
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| 关 键 词： | 东北扁核木 组织培养 快速繁育技术 |
Study on Rapid Propagation of Prinsepia sinensis Tissue Culture |
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| Abstract: | Objective] The aim was to screen out the optimal primary culture, subculture and rooting culture medium for Prinsepia sinensis tissue culture. Method] With single bud stem segment of Prinsepia sinensis as explant, using MS or 1/2 MS as the basic culture medium, supplemented with different concentrations of 6-BA, IBA, NAA and GA3 , effects of different culture mediums on bud induction, proliferation and rooting were discussed. Result] IBA concentration influenced significantly on starting rate of primary culture of Prinsepia sinensis, the or-der of influence degree was IBA concentration>GA3 concentration>6-BA concentration;6-BA concentration had obvious influence on prolif-eration coefficient of subculture medium, the order of influence degree was 6-BA concentration>IBA concentration>NAA concentration;IBA concentration had a significant effect on rooting index, while NAA concentration had no significant effect. Conclusion] The optimal primary culture, subculture and rooting culture mediums for Prinsepia sinensis tissue culture were: MS +0.5 mg/L 6-BA +0.2 mg/L IBA; MS +1.0 mg/L 6-BA+0.1 mg/L IBA;1/2 MS+2.0 mg/L IBA+0.1 mg/L NAA. |
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| Keywords: | Prinsepia sinensis Tissue culture Rapid propagation technology |
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