Diverse <Emphasis Type="Italic">Fusarium solani</Emphasis> isolates colonise agricultural environments in Ethiopia |
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| Authors: | M Bogale ET Steenkamp MJ Wingfield BD Wingfield |
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| Institution: | (1) Department of Genetics, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa;(2) Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa |
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| Abstract: | Fusarium solani is a fungal pathogen that infects many different genera of plants. It represents one of the two Fusarium spp. commonly isolated from agricultural soils and plant tissues in Ethiopia. To determine the diversity of F. solani in Ethiopia, we studied 43 isolates using Amplified Fragment Length Polymorphism (AFLP) and nucleotide sequences of the Translation
Elongation Factor 1α (TEF-1α) and β-tubulin genes. TEF-1α sequences from GenBank, representing previously described species
and clades of the F. solani-Haematonectria haematococca complex, were also included for comparative purposes. Phylogenetic analyses of the TEF-1α data separated the isolates into
three groups corresponding with the three previously described clades (Clades 1–3) for this fungus. The Ethiopian isolates
aggregated into one group corresponding to Clade 3. TEF-1α, β-tubulin and AFLPs further separated the Ethiopian isolates into
a number of clusters and apparently novel phylogenetic lineages. Although the biological and ecological significance of these
lineages and clusters is unclear, our data show that the Ethiopian agricultural environment is rich in species and lineages
of the F. solani-H. haematococca complex. |
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| Keywords: | AFLP β -tubulin Ethiopia Fusarium solani TEF-1α |
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