| 鸡恒定链活性片段和NDVF蛋白表位嵌合体的构建与鉴定 |
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| 引用本文： | 倪庆胜,陈芳芳,余为一.鸡恒定链活性片段和NDVF蛋白表位嵌合体的构建与鉴定[J].安徽农业科学,2012,40(25):12556-12557,12574. |
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| 作者姓名： | 倪庆胜 陈芳芳 余为一 |
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| 作者单位： | 安徽农业大学,安徽省人兽共患病重点实验室,安徽合肥230036 |
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| 基金项目： | 国家自然科学基金项目(31172306) |
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| 摘 要： | 目的]为研究基于恒定链(Ii)分子片段的免疫载体提供试验材料。方法]利用鸡恒定链的部分有免疫活性的片段作为免疫载体结构,构建含新城疫病毒F蛋白抗原片段的嵌合体。结果]成功构建了Ii-key/F306、Ii-key/F306/AP、Cyt/Ii-key/F306和Cyt/Ii-key/F306/AP 4个嵌合体。重组质粒能被诱导表达相应蛋白分子,而且通过纯化获得95%高纯度。结论]该试验中嵌合体的成功制备为动物免疫试验奠定了基础。
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| 关 键 词： | 鸡恒定链 新城疫病毒 融合蛋白 嵌合体 原核表达 纯化 |
Construction and Identification of Chimera Containing Active Segments of Chicken Invariant Chain and F Protein Epitope of NDV |
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| Institution: | NI Qing-sheng et al(Key Laboratory of Zoonoses of Anhui Province,Anhui Agricultural University,Hefei,Anhui 230036) |
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| Abstract: | Objective] The research aimed to provide experimental materials for studying the immune carrier based on the molecular segments of invariant chain(Ii).Method] Using partial segments with immune activity of chicken invariant chain as the structure of immune carrier,the chimera containing the segments of F protein antigen of newcastle disease virus(NDV) was constructed.Result] Four chimeras were successfully constructed,including Ii-key/F306,Ii-key/F306/AP,Cyt/Ii-key/F306 and Cyt/Ii-key/F306/AP.The recombinant plasmids could be induced to express the related protein molecules.And these protein molecules could be purified to 95% in purity.Conclusion] The preparation of these chimeras laid the foundation for performing vaccination test on animals. |
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| Keywords: | Chicken invariant chain NDV Fusion protein Chimera Prokaryotic expression Purification |
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