| 葡萄籽中多酚物质的超声提取及其抗氧化活性研究 |
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| 引用本文： | 张晓梅,刘晓芳,高云涛.葡萄籽中多酚物质的超声提取及其抗氧化活性研究[J].安徽农业科学,2012,40(25):12341-12342. |
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| 作者姓名： | 张晓梅 刘晓芳 高云涛 |
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| 作者单位： | 云南民族大学文化学院,云南昆明650500;国家民族-教育部民族药资源化学重点实验室,云南昆明650500;国家民族-教育部民族药资源化学重点实验室,云南昆明650500;云南民族大学化学与生物技术学院,云南昆明650500 |
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| 摘 要： | 目的]研究葡萄籽多酚的超声波提取条件和抗氧化活性。方法]以超声提取获得葡萄籽多酚,采用Fenton反应.OH体系研究抗氧化活性。结果]最佳提取工艺条件为:超声提取60 min,浓度75%乙醇,料液比为1∶20。在最佳条件下,多酚得率为10.71%。葡萄籽多酚提取物对Fenton反应羟自由基清除的IC50为0.031 g/L,0.093 g/L时清除率达最大,最大清除率为87.12%。结论]葡萄籽多酚超声提取具有良好的抗氧化活性。
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| 关 键 词： | 超声波 葡萄籽 多酚 提取 抗氧化 |
Ultrasonic Extraction of Polyphenols from Grape Seeds and Its Antioxidant |
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| Institution: | ZHANG Xiao-mei et al(School of Chemistry and Biotechnology,Yunnan University of Nationalities,Kunming,Yunnan 650500) |
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| Abstract: | Objective] The research aimed to study the ultrasonic extraction and antioxidant activity of polyphenols extracted from grape seeds.Method] Polyphenols of grape seeds was extracted by ultrasonic extraction and antioxidant activity was determined by scavenging effect on Fenton reaction ·OH radical with spectrophotometry.Results] The optimum condition for the extraction of polyphenols were as follows: 60 min of ultrasonic time,75% of alcohol and 1∶ 20 of solid-liquid ratio.An extraction rate for the polyphenols from grape seeds was 10.71% under the optimum condition.The maxium scavening rate of 0.093 g/L extraction on ·OH was 87.12 % and IC50 reached 0.031 g/L.Conclusion] The polyphenols from grape seeds obtained by ultrasonic extraction have good antioxidant activity. |
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| Keywords: | Ultrasonic Grape seeds Polyphenol Extraction Antioxidantion |
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