| 番茄灰霉病菌拮抗基因工程菌株Fh-chib培养条件的研究 |
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| 引用本文： | 韩文霞,李伟泽,陈立,陈五岭.番茄灰霉病菌拮抗基因工程菌株Fh-chib培养条件的研究[J].安徽农业科学,2012,40(25):12521-12523. |
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| 作者姓名： | 韩文霞 李伟泽 陈立 陈五岭 |
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| 作者单位： | 西安医学院,陕西西安,710021;中国科学院西安分院,陕西西安,710043;西北大学生命科学学院,陕西西安,710069 |
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| 摘 要： | 目的]筛选番茄灰霉病菌拮抗基因工程菌株Fh-chib的最佳培养条件。方法]在单因素试验的基础上,采用正交试验设计对番茄灰霉病菌拮抗菌株Fh-chib的最佳培养条件(碳源、氮源和无机盐)进行了研究。结果]工程菌株Fh-chib的最佳培养基配方为0.50%葡萄糖、1.00%蔗糖、1.00%酵母膏、1.50%麸皮、1.00%黄豆饼粉、0.03%MgSO4和0.40%CaCl2。各个因素对发酵液抑菌活性影响的主次顺序为:葡萄糖>蔗糖>CaCl2>黄豆饼粉>酵母膏>麸皮>MgSO4。结论]为该菌株的工业化生产奠定了基础。
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| 关 键 词： | 番茄灰霉病菌 芽孢杆菌Fh-chib 培养基 |
Studies on Fermentation Conditions of Antagonistic Actinomyle Fh-chib against Botrytis cinerea |
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| Institution: | HAN Wen-xia et al(Xi’an Medical University,Xi’an,Shaanxi 710021) |
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| Abstract: | Objective] The aim was to screen out the optimal culture conditions of antagonistic actinomyle Fh-chib against Botrytis cinerea.Method] Based on single factor experiment,the optimal culture conditions of antagonistic actinomyle Fh-chib against Botrytis cinerea were studied by using orthogonal experimental design method,mainly including carbon source,nitrogen source and inorganic salts.Result] The optimum medium for Fh-chib consisted of 0.50% glucose,1.00% sucrose,1.00% yeast extract,1.50% bran,1.00% soybean powder,0.03% MgSO4 and 0.40% CaCl2,and effects of every factors on the antibacterial activity of fermentation broth were in order of glucose > sucrose > CaCl2 >soybean powder > yeast extract > bran > MgSO4.Conclusion] The research lays the foundation for industrial production of Fh-chib. |
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| Keywords: | Botrytis cinerea Bacillus Fh-chib Medium |
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