| 核桃壳多糖的含量测定 |
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| 引用本文： | 周萍,罗延梦,马媛,黄海鹤,罗爱俊,唐龙.核桃壳多糖的含量测定[J].安徽农业科学,2012,40(25):12654-12655. |
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| 作者姓名： | 周萍 罗延梦 马媛 黄海鹤 罗爱俊 唐龙 |
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| 作者单位： | 大理学院药学与化学学院,云南大理,671000 |
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| 基金项目： | 云南省科技厅应用基础研究项目(2010ZC137);大理学院大学生科研基金项目(DXSKYX201003) |
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| 摘 要： | 目的]测定核桃壳多糖的含量。方法]以葡萄糖为对照品,采用苯酚-硫酸法在490 nm测定核桃壳中多糖含量。结果]最佳显色条件为5%苯酚1.0 ml,浓硫酸6.0 ml,反应温度65℃,反应时间为30 min,平均回收率为101.9%,RSD为1.98%,线性范围10～90μg/ml。结论]该方法简便、准确、重现性好,适用于核桃壳多糖的含量测定。
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| 关 键 词： | 核桃壳 多糖 提取 苯酚-硫酸法 |
Determination of the Content of Polysaccharide in Walnut Shell |
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| Institution: | ZHOU Ping et al(College of Pharmacy and Chemistry,Dali University,Dali,Yunnan 671000) |
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| Abstract: | Objective] To determine the content of polysaccharide in walnut shell.Method] The content of polysaccharide was determined by phenol-sulfuric acid method at 490 nm with the glucose as reference substance.Result] The optimal color conditions were 1.0 ml 5% phenol,6.0 ml sulfuric acid,65 ℃ reaction temperature and 30 min reaction time,the average recovery was 101.9%,RSD=1.98% and the liner range was 10-90 μg/ml.Conclusion] The method is simple,accurate and has good reproducibility,and it is reliable to determine the total polysaccharide in the walnut shell. |
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| Keywords: | Walnut shell Polysaccharides Extraction Pheno-sulfuric acid method |
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