| 三氧化二砷对大鼠成骨细胞增殖及BMP-2的影响 |
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| 引用本文： | 陆祥,范桂金,陈伟,何志全.三氧化二砷对大鼠成骨细胞增殖及BMP-2的影响[J].安徽农业科学,2012,40(24):12075-12078. |
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| 作者姓名： | 陆祥 范桂金 陈伟 何志全 |
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| 作者单位： | 遵义医学院基础医学院,贵州遵义,563000 |
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| 基金项目： | 遵义医学院硕士启动基金项目(F-351) |
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| 摘 要： | 目的]观察不同剂量三氧化二砷(As2O3)对体外培养的大鼠成骨细胞增殖、骨形成蛋白-2(BMP-2)基因表达的影响。方法]不同浓度(10、1、0.1μmol/L)的三氧化二砷作用于体外培养的成骨细胞48和72 h后,观察成骨细胞的形态,采用MTT法检测成骨细胞的增殖,并通过RT-PCR检测成骨细胞BMP-2基因的表达情况。结果]10μmol/L As2O3组可见部分成骨细胞变圆变小,细胞核固缩、碎裂,细胞膜皱褶、凹陷;1μmol/L As2O3组成骨细胞数目众多和分裂相明显;0.1μmol/L As2O3组成骨细胞间连接紧密。10μmol/LAs2O3组大鼠成骨细胞OD值明显低于对照组,而72 h OD值低于48 h OD值,有显著性差异(P0.01);1μmol/L组As2O3OD值明显高于对照组,而72 h OD值高于48 h OD值,有显著性差异(P0.01);0.1μmol/L As2O2组72 h OD值与48 h OD值无显著差异(P0.05),且OD值与对照组无显著差异(P0.05)。RT-PCR结果表明,10μmol/L组72 h BMP-2基因表达明显低于48 h,且BMP-2的表达明显低于对照组,有显著性差异(P0.01);1μmol/L As2O3组72 h BMP-2基因表达与48 h无显著差异(P0.05),且BMP-2基因表达与对照组(P0.05);0.1μmol/L As2O3组72 h BMP-2基因表达明显高于48 h,且BMP-2的表达明显高于对照组,有显著性差异(P0.01)。结论]As2O3对体外培养大鼠成骨细胞增殖和BMP-2 mRNA表达具有双向调节作用,并且呈剂量-时间依赖性。
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| 关 键 词： | AsO 成骨细胞 增殖 BMP- |
Effects of Arsenic Trioxide on the Proliferation and BMP-2 in Osteoblasts Cultured in vitro |
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| Institution: | LU Xiang et al(Zunyi Medical University,Zunyi,Guizhou 563000) |
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| Abstract: | Objective] To study the effects of different doses of arsenic trioxide on the proliferation and bone morphogenetic protein-2(BMP-2) gene expression of cultured rat osteoblasts cells.Method] After the osteoblasts were treated by different concentrations of As2O3(10,1 and 0.1μmol/L) for 48 h and 72 h,the morphology of osteoblasts was observed under inverted microscophy,the proliferation of osteoblasts was tested by MTT assay,and the BMP-2 gene expression of osteoblasts was determined with RT-PCR.Result] In 10 μmol/L arsenic trioxide treatment group,some osteoblasts become rounder and smaller,the nuclear was condensed and fragmented,and the cell membrane folded and depressed;in 1 μmol/L arsenic trioxide treatment group,there were large number of osteoblasts with obvious split phase;in 0.1 μmol/L arsenic trioxide treatment group,the osteoblasts were closely connected.The OD value of 10 μmol/L group was significantly lower than the control group,OD value in 72 h was lower than 48 h,which had statistical difference(P<0.01);OD value of 1 μmol/L group was significantly higher than the control group.OD value in 72 h was higher than OD value in 48 h,which had statistical difference(P<0.01).The OD value of 0.1 μmol/L group in 72 h had no statistical difference with 48 h(P>0.05) and was the same as control group.The BMP-2 gene expression of 10 μmol/L group in 72 g was significantly lower than in 48 g and BMP-2 gene expression of 10 μmol/L group was significantly lower than the control group,which had statistical difference(P<0.01);BMP-2 gene expression of 1 μmol/L group had no significant difference between in 48 h and in 72 h and the same as control group(P>0.05).BMP-2 gene expression of 0.1 μmol/L group in 72 h was significantly higher than in 48 h and BMP-2 gene expression of 10 μmol/L group was significantly higher than the control group,which had statistical difference(P<0.01).Conclusion] As2O3 has a positive-negative regulatory role for cell proliferation and BMP-2 mRNA expression to rat osteoblast in vitro,and presents a dose-time dependence. |
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| Keywords: | Arsenic trioxide Osteoblasts Proliferation BMP-2 |
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