| 不同激素配比对三岛柴胡悬浮细胞生长发育的影响 |
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| 引用本文： | 张东向,徐爽,史绍林,矫艳玲.不同激素配比对三岛柴胡悬浮细胞生长发育的影响[J].安徽农业科学,2012,40(26):12864-12865,12895. |
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| 作者姓名： | 张东向 徐爽 史绍林 矫艳玲 |
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| 作者单位： | 齐齐哈尔大学,黑龙江齐齐哈尔,161006;齐齐哈尔大学,黑龙江齐齐哈尔,161006;齐齐哈尔大学,黑龙江齐齐哈尔,161006;齐齐哈尔大学,黑龙江齐齐哈尔,161006 |
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| 基金项目： | 黑龙江省自然科学基金资助项目(C201013) |
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| 摘 要： | 目的]研究不同配比激素对三岛柴胡悬浮细胞生长发育的影响。方法]采用均匀设计试验方法,对三岛柴胡悬浮细胞培养基的激素进行优化,并以最优激素配方为基础拟合三岛柴胡悬浮细胞培养中鲜重、干重与培养时间的关系曲线。结果]三岛柴胡悬浮细胞培养的最优激素配方为:N6+0.5 mg/L NAA+0.75 mg/L 6-BA+1.5 mg/L KT;细胞鲜重与培养时间的关系拟合方程为Y=5.703 6X2-41.429X+136.44;细胞干重与培养时间的关系拟合方程为Y=2.021 8X2-22.595X+57.19。结论]试验为研究三岛柴胡细胞和实现其大规模培养奠定了基础。
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| 关 键 词： | 三岛柴胡(Bupleurum Falcatum L.) 激素优化 悬浮细胞培养 |
The Effects of Different Hormones on the Suspension Cell Growth and Development of Bupleurum Falcatum L. |
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| Institution: | ZHANG Dong-xiang et al(Qiqihar University,Qiqihar,Heilongjiang 161006) |
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| Abstract: | Objective] To study the effects of different hormones the suspension cell growth and development of Bupleurum Falcatum L..Method] The plant growth hormone of medium for Bupleurum Falcatum L.suspension cell cultures was optimized by the test method of uniform design.Result] The optimal hormone formulation of B.falcatum suspension cell cultures was N6+0.5 mg/L NAA+0.75 mg/L 6-BA+1.5 mg/L KT.Based on the optimal hormone formulation to fit the curves about the relationship of the cell fresh-weight t and the cell dry-weight in incubation time,the fitting equation of the relationship between the cell fresh-weight and the incubation time was Y=5.703 6X2-41.429X+136.44;the fitting equation of the relationship between the cell dry-weight and the incubation time was Y=2.021 8X2-22.595X+57.19.Conclusion] This study can lay the foundation for the further study and the large-scale culture about the B.falcatum L.. |
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| Keywords: | Bupleurum Falcatum L Hormone optimization Suspension cell culture |
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