| 作物病害高效生防菌株的定向快速筛选及其种类鉴定 |
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| 引用本文： | 石磊,郭庆港,李宝庆,鹿秀云,李社增,王洪港,马平.作物病害高效生防菌株的定向快速筛选及其种类鉴定[J].安徽农业科学,2012,40(24):12068-12071,12096. |
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| 作者姓名： | 石磊 郭庆港 李宝庆 鹿秀云 李社增 王洪港 马平 |
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| 作者单位： | 河北农业大学植物保护学院,保河北定071000;河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000;河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000;河北省黄骅市城市管理局,河北黄骅,061104 |
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| 基金项目： | 现代农业产业技术体系建设专项资金项目(CARS-18-15);国家自然科学基金项目(30900962);“十二五”农村领域国家科技计划课题(2011AA10A205) |
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| 摘 要： | 目的]筛选和鉴定能产Bacillomycin D的芽孢杆菌。方法]通过PCR技术对36株具有抑菌活性的芽孢杆菌进行检测,筛选出具有Bacillomycin D合成酶基因的菌株,并利用快速蛋白液相色谱技术(FPLC)对筛选出的菌株进行检测,最后通过16S rDNA和gyrB基因序列对这些菌株进行分子鉴定。结果]共筛选出4株能产生Bacillomycin D的菌株,其中2株为萎缩芽孢杆菌(Bacillus atrophaeus),2株为解淀粉芽孢杆菌(B.amyloliquefaciens)。结论]该研究为高效微生物农药的开发奠定了基础。
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| 关 键 词： | 拮抗细菌 脂肽类抗生素 芽孢杆菌 筛选 鉴定 |
Fast Screening and Identification of High Efficiency Biocontrol Strains against Plant Disease |
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| Institution: | SHI Lei et al(College of Plant Protection,Agricultural University of Hebei,Baoding,Hebei 071000) |
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| Abstract: | Objective] The aim was to screen and identify Bacillomycin D producing strains of Bacillus.Method] PCR technique was used for detection the potential Bacillomycin D producing strains from 36 Bacillus wild type strains,and the isolated strains were detected by FPLC.Finally,the 16S rDNA and gyrB sequences were used to identify the Bacillomycin D producing strains.Result] Four Bacillomycin D producing strains were selected,and strain CJT-2 and BNT-34 were identified as B.amyloliquefaciens,XJT-7 and CAB-1 were identified as B.atrophaeus.Conclusion] The study lays a foundation for development of high efficiency microbial pesticides. |
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| Keywords: | Antagonistic bacteria Lipopeptide antibiotic Bacillus Screening Identification |
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