| Polyploid induction of Lespedeza formosa by colchicine treatment |
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| 作者姓名： | Li Wei Hu Dong-nan Li Hui Chen Xiao-yang |
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| 作者单位： | Li Wei1 Hu Dong-nan2 Li Hui1 Chen Xiao-yang1 1College of Biological Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，P. R. China 2College of Landscape Architecture and Art，Jiangxi Agriculture University，Nanchang 330045，P. R. China |
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| 摘 要： | Polyploid induction has been conducted by different colchicines concentrations with seed, hypocotyl and apices of Lespe-deza formosa. Morphological variation and amounts of chromosome about the polyploidy have also been analyzed. Results show that the best material for polyploid induction of L. formosa is the new apices from seed germination, with an induction rate of 44.4% at 0.1% colchicines concentration and 36 hours of treatment time. Compared with normal diploid plants, the polyploidy plants of L. for-mosa inducted in our experiments have short stature and stems with thick and wide leaves. Cytological studies show that the changed seedlings, whose morphology has changed dramatically, are tetraploid, with 44 (2n = 4x = 44) chromosomes in the somatic cells.
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| 关 键 词： | 胡枝子属 秋水仙素 植物病害 林业 |
| 收稿时间： | 2007-05-10 |
| 修稿时间： | 2007-08-15 |
Polyploid induction of <Emphasis Type="Italic">Lespedeza formosa</Emphasis> by colchicine treatment |
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| Li Wei Hu Dong-nan Li Hui Chen Xiao-yang.Polyploid induction of Lespedeza formosa by colchicine treatment[J].Forestry Studies in China,2007,9(4):283-286. |
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| Authors: | Li Wei Hu Dong-nan Li Hui Chen Xiao-yang |
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| Institution: | 1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083. P. R. China
2. College of Landscape Architecture and Art,Jiangxi Agriculture University, Nanchang 330045,P. R. China |
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| Abstract: | Polyploid induction has been conducted by different colchicines concentrations with seed, hypocotyl and apices of Lespe- deza formosa. Morphological variation and amounts of chromosome about the polyploidy have also been analyzed. Results show that the best material for polyploid induction of L. formosa is the new apices from seed germination, with an induction rate of 44.4% at 0.1% colchicines concentration and 36 hours of treatment time. Compared with normal diploid plants, the polyploidy plants of L. for- mosa inducted in our experiments have short stature and stems with thick and wide leaves. Cytological studies show that the changed seedlings, whose morphology has changed dramatically, are tetraploid, with 44 (2n = 4x = 44) chromosomes in the somatic cells. |
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| Keywords: | Lespedeza formosa colchicincs polyploid induction morphological variation |
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