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Mass-marking of grayling, Thymallus thymallus (L.), larvae by fluorochrome tagging of otoliths
Authors:M NAGIEC  P CZERKIES  K GORYCZKO  A WITKOWSKI  E MURAWSKA
Institution:Olsztyn University of Agriculture and Technology, Olsztyn-Kortowo;Inland Fisheries Institute, Salmonid Research Laboratory, Zukowo;Museum of Natural History, Wroclaw University, Wroclaw, Poland
Abstract:Abstract Larvae of grayling, Thymallus thymallus (L.) were immersed in tetracycline hydrochloride (TC) and alizarin red S (AL) solutions for 3–4 h at concentrations of 300 and 70 ppm, respectively. Fluorescent marks on the otoliths from the marked fish were identified using a UV-light microscope. Results obtained with AL were superior to TC in marking otoliths. Fish kept in an aquarium retained good marks for 842 days. Stocking of the Carpathian River Ochotnica with marked grayling larvae revealed good mark retention for 128 days and a survival rate of approximately 12.2%.
Keywords:Alizarin  grayling  immersion  marking longevity  mass-marking  otolith  survival rate  tetracycline
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