| 传染性法氏囊病病毒强毒株HB／11的分离与鉴定 |
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| 引用本文： | 黄显明,张小飞,丁美娟,尹秀凤,许秀梅,王伟,靳宇田.传染性法氏囊病病毒强毒株HB／11的分离与鉴定[J].中国动物传染病学报,2012,20(5):8-11. |
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| 作者姓名： | 黄显明 张小飞 丁美娟 尹秀凤 许秀梅 王伟 靳宇田 |
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| 作者单位： | 南京天邦生物科技有限公司,南京,211102 |
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| 摘 要： | 2011年8月，从河南省疑似传染性法氏囊病鸡群采集病料，通过鸡胚接种、琼脂扩散试验和RT-PCR等方法，证实分离的病毒为传染性法氏囊病病毒（Infectiousbursaldiseasevirus，IBDV），命名为HB／11株。序列分析表明，HB／11株VP2基因的核苷酸序列与GenBank中发表的部分国内外IBDV超强毒株VP2基因序列相似性在99％左右；HB／11株VP2高变区基因含有七肽基序为SWSASGS，在222、253、256、279、284、294和299位上的氨基酸残基分别是A、Q、I、D、A、I和S，具有IBDV强毒的分子特征。动物回归试验表明，30日龄鸡群接种该毒株后引起鸡群发病率为100％，死亡率为70％，剖检可见鸡传染性法氏囊病典型病变。因此该病毒为IBDV强毒株。
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| 关 键 词： | 传染性法氏囊病病毒 分离鉴定 VP2基因 |
ISOLATION AND IDENTIFICATION OF VIRULENT STRAIN HB/ll OF INFECTIOUS BURSAL DISEASE VIRUS |
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| HUANG Xian-ming,ZHANG Xiao-fei,DING Mei-juan,YIN Xiu-feng,XU XIU-mei,WANG Wei,JIN Yu-tian.ISOLATION AND IDENTIFICATION OF VIRULENT STRAIN HB/ll OF INFECTIOUS BURSAL DISEASE VIRUS[J].Chinese Journal of Animal Infectious Diseases,2012,20(5):8-11. |
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| Authors: | HUANG Xian-ming ZHANG Xiao-fei DING Mei-juan YIN Xiu-feng XU XIU-mei WANG Wei JIN Yu-tian |
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| Institution: | (Nanjing Tech-bank Bio-industry Co.,LTD,Nanjing 210012,China) |
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| Abstract: | Infectious bursal disease virus(IBDV) HB/11 strain was isolated in August 2011 from diseased chickens in Henan province and confirmed in embryo inoculation,agar gel diffusion and RT-PCR.Sequence analysis showed that VP2 gene of HB/11 strain shared about 99% homology with some very virulent IBDV strains deposited in GenBank.The HB/11 strain had the heptapeptide motif(SWSASGS) and critical amino acid residues A222,Q253,I256,D279,A284,I294 and S299 in the variable region of VP2 gene,suggesting that it possessed the molecular characteristics of virulent IBDV.Thirty-day old chickens were inoculated with the isolate.The infected chickens developed clinical signs and pathological changes typical of IBD,and showed 100% morbidity and 70% mortality. |
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| Keywords: | Infectious bursal disease virus (IBDV) isolation and identification VP2 gene |
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