Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand |
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| Authors: | Tawatchai Pohuang Niwat Chansiripornchai Achara Tawatsin Jiroj Sasipreeyajan |
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| Institution: | Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. |
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| Abstract: | Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and amino acid sequence identities less than 84% in common with IBVs published in the GenBank database. This group likely represents the strains indigenous to Thailand. The isolates in group II showed a close relationship with Chinese IBVs. They had nucleotide sequence identities of 97-98% and amino acid sequence identities 96-98% in common with Chinese IBVs (strain A2, SH and QXIBV). This finding indicated that the recent Thai IBVs evolved separately and at least two groups of viruses are circulating in Thailand. |
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| Keywords: | infectious bronchitis virus phylogenetic analysis S1 gene sequencing Thailand |
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