| 水貂犬瘟热Vero细胞活疫苗(CDV3-CL株,悬浮培养)临床安全性和免疫效力评价 |
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| 引用本文： | 冯二凯,易立,陈立志,张 淼,罗国良,郭 利,王振军,程世鹏,任 飞,程悦宁.水貂犬瘟热Vero细胞活疫苗(CDV3-CL株,悬浮培养)临床安全性和免疫效力评价[J].中国兽药杂志,2020,54(4):24-29. |
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| 作者姓名： | 冯二凯 易立 陈立志 张 淼 罗国良 郭 利 王振军 程世鹏 任 飞 程悦宁 |
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| 作者单位： | 中国农业科学院特产研究所 长春,;吉林特研生物技术有限责任公司 长春,,,,,,,,,中国农业科学院特产研究所 长春 |
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| 基金项目： | 吉林省“双十项目”重大科技攻关(20150201006NY)；吉林省科技发展计划(20150204073NY) |
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| 摘 要： | 为评价水貂犬瘟热Vero细胞活疫苗(CDV3-CL株,悬浮培养)冻干制剂在临床应用中的安全性和免疫效果,分别在吉林、辽宁两省选择饲养规模相似的3个毛皮兽场进行疫苗安全性(10头份)和免疫效力实验(1头份)研究,接种后观察动物的临床症状,检测抗体水平并随机选择部分水貂进行攻毒试验。结果显示,超剂量接种水貂未见有明显的临床症状,对水貂的生产性能无明显影响;水貂免疫疫苗后21 d即可获得达到免疫保护的抗体水平;免疫180 d仍能维持较高水平的抗体,且能够保护动物抵抗强毒攻击。试验表明,水貂犬瘟热Vero细胞活疫苗(CDV3-CL株,悬浮培养)冻干制剂具有较好的临床保护效果。
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| 关 键 词： | 水貂 犬瘟热 活疫苗 悬浮培养 临床试用 |
| 收稿时间： | 2019/12/25 0:00:00 |
| 修稿时间： | 2020/4/7 0:00:00 |
Evaluation on the Clinical Immune Effects and Safety of Mink Canine Distemper Virus Attenuated Lived Vaccine in Field Trial |
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| FENG Er-kai,YI Li,CHEN Li-zhi,ZHANG Miao,LUO Guo-liang,GUO Li,WANG Zhen-jun,CHENG Shi-peng,REN Fei,CHENG Yue-ning.Evaluation on the Clinical Immune Effects and Safety of Mink Canine Distemper Virus Attenuated Lived Vaccine in Field Trial[J].Chinese Journal of Veterinary Drug,2020,54(4):24-29. |
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| Authors: | FENG Er-kai YI Li CHEN Li-zhi ZHANG Miao LUO Guo-liang GUO Li WANG Zhen-jun CHENG Shi-peng REN Fei CHENG Yue-ning |
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| Institution: | Institution of Special Animal and Plant Science of Chinese Academy of Agriculture Science;China;Jiling Teyan Biotechnology Co,Ltd;China,,,,,,,,, |
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| Abstract: | Clinical safety(10 dose)and immune efficacy(1 dose)evaluation of the mink canine distemper attenuated live vaccine(CDV3-CL strain,suspension)have been conducted in 3 fur animal farms(one in Liaoning province,two were in Jilin provinces),respectively.During the whole period of the clinical safety trials,all vaccinated animals were clinically normal as non-immunized controls.At the onset of immune efficacy tests,animals were vaccinated with one dose of newly prepared vaccine,and sera were collected on 7,14,21,60,120 and 180 days post immunization(dpi)for test CDV serum neutralizing antibody(SN).The results showed that the SN could be detected as early as 7 dpi and it could reach to a high level(≥1∶64)at 21 dpi,which enough to protect mink from a challenge of 100 ID50 virulent CDV-LNM strain.The period of immunity duration is over 180 d.The above results demonstrated that this vaccine could be widely used in practice. |
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| Keywords: | mink canine distemper virus lived vaccine suspension filed trail |
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