| 荞麦愈伤组织的高频率诱导和植株再生研究 |
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| 引用本文： | 王茅雁,锡建中,张晶,边翠茹.荞麦愈伤组织的高频率诱导和植株再生研究[J].内蒙古农业大学学报(自然科学版),2000,21(2):41-44. |
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| 作者姓名： | 王茅雁 锡建中 张晶 边翠茹 |
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| 作者单位： | 1. 内蒙古农业大学生物工程系,呼和浩特,010018
2. 内蒙古林业厅,呼和浩特,010010 |
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| 摘 要： | 研究了高频率诱导荞麦愈伤组织和再生植株的组培技术。在附加０．５ｍｇ／Ｌ ２．４－Ｄ和ＫＴ的ＭＳ４培养基上，胚轴和子叶外植体愈伤组织的诱导率高达８１．３％￣９１．２％，胚轴不定芽的诱导率为３４．５％￣４１．２％。将胚轴在附加０．５ｍｇ／Ｌ ２．４－Ｄ的ＭＳ２上预培养１５ｄ左右再转到ＭＳ４上，不定芽诱导率可达５３．３％。不定芽也可从培养于ＭＳ５（附加０．５ｍｇ／Ｌ ＫＴ）中的外植体上直接产生，当不定芽转到附加０．５ｍｇ／Ｌ ＮＡＡ的ＭＳ７培养基上可诱导生根。本研究为进一步开展荞麦细胞工程和基因工程研究提供了较成熟的组培技术。
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| 关 键 词： | 荞麦 胚轴 子叶 愈伤组织 植株再生 高频率诱导 |
| 修稿时间： | 1999-12-13 |
HIGH FREQUENCY INDUCTION OF CALLUS AND PLANT REGENERATION IN BUCKWHEAT |
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| WANG Mao-yan,XI Jian-zhong,ZHANG Jing,BIAN Cui-ru.HIGH FREQUENCY INDUCTION OF CALLUS AND PLANT REGENERATION IN BUCKWHEAT[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2000,21(2):41-44. |
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| Authors: | WANG Mao-yan XI Jian-zhong ZHANG Jing BIAN Cui-ru |
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| Abstract: | Methods for high frequency induction of callus and regenerated plant of backwheat were studied.On the MS medium containing 0.5mg/L 2.4-D and 0.5mg/L KT,named MS4,the rate of callus induced from epicotyl and cotyledon explants was 81.3%-91.2%,and the rate of adventitious shoots from epicotyl was between 34.5%-41.2%,If epicotyl explants were precultured about for 15 days on MS with 0.5mg/L 2.4-D prior to be transfered to MS4,the rate of shoots induced could rise to 53.3%.Shoots also emersed derectly from the explants cultured on the MS medium containing 0.5mg/L KT.After having been transfered to the MS7 with 0.5mg/L NAA,shoots rooted.This experiment will provide a ripe culture method for further studies on cell engineering and genetic engineering in buckwheat. |
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| Keywords: | buckwheat epicotyl cotyledon callus plant regeneration |
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