| 猪嵴病毒RT-PCR检测方法的建立及应用 |
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| 引用本文： | 张文波,冷闯,段俊,邓舜洲.猪嵴病毒RT-PCR检测方法的建立及应用[J].中国畜牧兽医,2013,40(1):42-46. |
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| 作者姓名： | 张文波 冷闯 段俊 邓舜洲 |
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| 作者单位： | 1. 江西农业大学动物科学技术学院,江西南昌,330045
2. 南昌金牧动物保健服务有限公司,江西南昌,330013 |
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| 基金项目： | 国家自然科学基金项目(31160498/C1803) |
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| 摘 要： | 根据GenBank中登录的猪嵴病毒3D基因保守序列,设计合成1对引物,以腹泻哺乳仔猪的小肠为起始材料,建立了猪峭病毒的RT-PCR检测方法,并用该方法对收集的50份哺乳仔猪腹泻样本进行检测。结果显示,该方法对模板的最小检测量是2.3 pg,检测猪其他常见致腹泻病毒均为阴性,同一样品3次试验结果一致;所扩增的序列经测序分析均为猪嵴病毒的3D核苷酸序列;仔猪腹泻样本的猪嵴病毒检出率达58%。结果表明,该方法敏感度高、重复性和特异性良好,可用于猪嵴病毒的分子流行病学调查和临床病例的快速诊断。
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| 关 键 词： | 猪嵴病毒 RT-PCR 同源性分析 |
| 收稿时间： | 2012-06-26 |
Establishment and Application of RT-PCR Detection of Pocrine Kobuvirus |
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| ZHANG Wen-bo
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LENG Chuang
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DUAN Jun
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DENG Shun-zhou.Establishment and Application of RT-PCR Detection of Pocrine Kobuvirus[J].China Animal Husbandry & Veterinary Medicine,2013,40(1):42-46. |
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| Authors: | ZHANG Wen-bo LENG Chuang DUAN Jun DENG Shun-zhou |
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| Institution: | 1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;2. Nanchang Jinmu Animal Health Services Co., Ltd., Nanchang 330013, China |
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| Abstract: | A detection method for porcine kobuvirus (PKV) with RT-PCR technique was established to detect the 3D gene of PKV from the intestinal tract of diarrhea piglets, with the detection limit about 2.3 pg RNA. 50 samples of diarrhea piglets were detected by the RT-PCR method, the PKV positive rate of samples was 58 percent. The PCR detection method established in this study has the characteristics of sensitivity, high specificity and good repeatability, and could be used in molecular epidemiological investigation and rapid clinical diagnosis of PKV. |
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| Keywords: | porcine kobuvirus RT-PCR homology analysis |
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