Detection of Bartonella sp. and a novel spotted fever group Rickettsia sp. in Neotropical fleas of wild rodents (Cricetidae) from Southern Brazil |
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| Institution: | 1. Centro de Pesquisa em Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Eldorado do Sul, RS, Brazil;2. Área de Vida Assessoria e Consultoria em Biologia e Meio Ambiente, Canoas, RS, Brazil;3. Universidade Luterana do Brasil, Canoas, RS, Brazil;4. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Jaboticabal, SP, Brazil;5. Museu de Ciências Naturais (MCN), Fundação Zoobotânica do Rio Grande do Sul (FZB-RS), Porto Alegre, RS, Brazil;1. Department of Animal Biology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil;2. Department of Genetics and Evolution, Microbiology and Immunology, Biology Institute, State Campinas University, Campinas, São Paulo, Brazil;3. Superintendência de Controle de Endemias, Seção Técnica de Pesquisa, Controle deVetores, Campinas, São Paulo, Brazil;4. Department of Clinical Pathology, Faculty of Medical Sciences, University of Campinas, São Paulo, Brazil;1. Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;2. Department of Virology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;3. Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;1. Comité Nacional de Investigaciones Científicas y Técnicas (CONICET), Ministerio de Ciencia, Tecnología e Innovación Productiva, Buenos Aires, Argentina;2. Instituto Nacional de Medicina Tropical (INMeT), ANLIS, Ministerio de Salud y Desarrollo Social de la Nación, Jujuy y Neuquén s/n, 3370, Puerto Iguazú, Misiones, Argentina;3. Departamento de Parasitologia, Instituto de Ciéncias Biológicas, Universidade Federal de Minas Gerais, Av. Pres. A. Carlos 6627, 31270-901, Belo Horizonte, MG, Brazil;4. Centro de Estudios Parasitológicos y de Vectores (CEPAVE) (CONICET CCT La Plata-UNLP), Boulevard 120 s/n e/60 y 64, 1900, La Plata, Argentina;1. Serviço de Referência Nacional em Vetores das Riquetsioses, Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Av. Brasil, 4365, Pav. Lauro Travassos, anexo posterior/sala 08, Manguinhos, Rio de Janeiro, RJ, CEP: 21045-900, Brazil;2. Centro Estadual de Vigilância em Saúde, Porto Alegre, Rio Grande do Sul, Brazil;3. Ministério da Saúde, Secretaria de Vigilância em Saúde, Unidade Técnica de Vigilância de Zoonoses, Brasília, Distrito Federal, Brazil;1. Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan;2. Laboratory of Veterinary Food Hygiene, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan;3. Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan;4. Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan;5. Hikiiwa Park Center, 1629 Inari-cho, Tanabe 646-0051, Japan |
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| Abstract: | The Neotropical region shows a great diversity of fleas, comprising more than 50 genera. The importance of the study of fleas is linked to their potential role as disease vectors. The aim of this study is to investigate the presence of Rickettsia spp. and Bartonella spp. in Neotropical fleas collected from wild rodents in Southern Brazil. From 350 rodents captured, 30 were parasitized by fleas. A total of 61 fleas belonging to two genera and six different species were collected (Craneopsylla minerva minerva, Polygenis occidentalis occidentalis, Polygenis platensis, Polygenis pradoi, Polygenis rimatus, and Polygenis roberti roberti). In 13 % of fleas of three different species (C. minerva, P. platensis, and P. pradoi) Rickettsia sp. DNA was found. Phylogenetic analysis of concatenated sequences of gltA, htrA, and ompA genes showed that Rickettsia sp. found in rodent fleas (referred as strain Taim) grouped together with Spotted Fever Group Rickettsia. In reference to Bartonella spp., five genotypes were identified in seven fleas of two species (C. minerva and P. platensis) and in five rodent spleens. Also, 207 frozen samples of wild rodents were screened for these pathogens: while none was positive for Rickettsia spp.; five rodent spleens were PCR-positive for Bartonella spp.. Herein, we show the detection of potential novel variants of Bartonella sp. and Rickettsia sp. in fleas collected of wild rodents from Southern Brazil. Further studies are needed to fully characterize these microorganisms, as well as to improve the knowledge on the potential role of Neotropical flea species as diseases vectors. |
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| Keywords: | Flea-borne Bartonellosis Zoonosis Pampa biome |
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