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四倍体刺槐扦插生根过程中氧化酶活性的变化
引用本文:马振华,赵 忠,张晓鹏.四倍体刺槐扦插生根过程中氧化酶活性的变化[J].西北农林科技大学学报(社会科学版),2007,35(7):85-89.
作者姓名:马振华  赵 忠  张晓鹏
作者单位:1. 西北农林科技大学林学院,陕西,杨凌,712100
2. 杨凌职业技术学院,林学系,陕西,杨凌,712100
3. 中国林业科学研究院,北京,100091
基金项目:国家林业局重点基础研究发展规划项目
摘    要:四倍体刺槐(Robinia pseudoacacia)属难生根树种,为了解其生根过程中氧化酶活性的变化规律,用300,400和500 mg/L的吲哚丁酸(IBA)处理四倍体刺槐嫩枝插条,以清水浸泡为对照,测定扦插生根过程中体内吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)和过氧化物酶(POD)活性的变化。结果表明,各IBA处理和对照插穗IAAO和POD活性变化趋势相似,在四倍体刺槐生根过程中呈升-降-升-降的变化规律,而PPO酶则基本呈降-升-降-升的变化规律;不同处理间3种酶活性的平均值和各阶段酶活性差异均显著。说明在四倍体刺槐扦插生根过程中,不定根的发生和发展与3种酶活性有密切联系,不同浓度IBA处理对各酶活性影响显著,其中以500 mg/L IBA处理对四倍体刺槐扦插生根的促进效果更明显。

关 键 词:四倍体刺槐  扦插生根  吲哚乙酸氧化酶  多酚氧化酶  过氧化物酶
文章编号:1671-9387(2007)07-0085-05
收稿时间:2006/11/12 0:00:00
修稿时间:2006-11-12

Studied on the oxidation enzymes activities in the periods of making roots in the green branch of Robinia pseudoacacia
MA Zhen-hua,ZHAO Zhong,ZHANG Xiao-peng,ZHANG Bo-yong,ZHAO Hui,ZHAN Xiao-hong.Studied on the oxidation enzymes activities in the periods of making roots in the green branch of Robinia pseudoacacia[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(7):85-89.
Authors:MA Zhen-hua  ZHAO Zhong  ZHANG Xiao-peng  ZHANG Bo-yong  ZHAO Hui  ZHAN Xiao-hong
Institution:1 College of Forestry,Northwest A & F University,Yangling,Shaanxi 712100,China;2.Department of Forestry,Yangling Vocational College of Technology,Yangling,Shaanxi 712100,China;3 Chinese Academy of Forestry,Beijing 100091,China)
Abstract:The Robinia pseudoacacia is a kind of trees with difficult growing roots.The green branch of Robinia pseudoacacia rootings are treated with the indoleacetic butyric acid(IBA),then the activities of indoleacetic acid oxidase(IAAO),polyphenol oxidase(PPO) and peroxidase(POD) are tested regularly.During the different periods of taking roots,the three enzymes activities vary differently;the indoleacetic acid oxidase(IAAO) and the peroxidase(POD) vary alike;the polyphenol oxidase(PPO) activities are contrary to the indoleacetic acid oxidase(IAAO) and peroxidase(POD).Besides,in the different periods of taking roots,the three enzymes activities show significant difference,and the different process of the enzymes activities also have remarkable difference,and the 500 mg/L of indoleacetic butyric acid(IBA) has the best marked improvement on the making roots of the Robinia pseudoacacia.
Keywords:Robinia pseudoacacia  rooting of cuttings  indoleacetic acid oxidase  polyphenol oxidase  peroxidase
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