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| H1N1亚型猪流感病毒的生物学特性测定及其HA基因序列分析 | |
| 引用本文： | 马祥,顾万军,刘镇明,黄良宗,彭启明.H1N1亚型猪流感病毒的生物学特性测定及其HA基因序列分析[J].动物医学进展,2006,27(9):59-62. |
| 作者姓名： | 马祥 顾万军 刘镇明 黄良宗 彭启明 |
| 作者单位： | 1. 华南农业大学兽医学院,广东广州,510642;佛山科技学院,广东佛山,528231 2. 佛山科技学院,广东佛山,528231 3. 华南农业大学兽医学院,广东广州,510642 |
| 摘 要： | 测定了A/Swine/Guangdong/1/01(H1N1)猪流感病毒的部分生物学特性;运用RT-PCR方法扩增其HA基因,并对其进行了克隆和序列分析。结果表明,该株病毒的EID50/0.2 mL及TCID50/0.1 mL分别为10-2.3和10-2.1,小鼠感染试验亦说明该病毒具有致病性。序列分析表明,HA基因全长为1 701 bp,与国内外流行株同源性达90.8%~99.0%。 |
| 关 键 词： | 猪流感 逆转录-聚合酶链反应 鸡胚半数感染量 半数的胞感染量 |
| 文章编号： | 1007-5038（2006）09-0059-04 |
| 收稿时间： | 2006-03-16 |
| 修稿时间： | 2006年3月16日 |
Study on Biological Character of Subtype H1N1 Swine influenza virus and Sequence Analysis of HA Gene |
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| MA Xiang,GU Wan-jun,LIU Zhen-ming,HUANG Liang-zong,PENG Qi-ming.Study on Biological Character of Subtype H1N1 Swine influenza virus and Sequence Analysis of HA Gene[J].Progress In Veterinary Medicine,2006,27(9):59-62. | |
| Authors: | MA Xiang GU Wan-jun LIU Zhen-ming HUANG Liang-zong PENG Qi-ming |
| Institution: | 1. College of Veterinary Medicine ,South China Agriculture University ,Guangzhou,Guangdong, 510642,China; 2. Foshan University, Foshan, Guangdong , 528231, China |
| Abstract: | The biological character of A/Swine/GuangDong/1/01(H1N1) SIV was studied in this article.The HA gene of SIV was amplified by RT-PCR,then cloned and sequenced to analysis.The result indicated that the EID_(50)/0.2mL and the TCID_(50)/0.1mL of this sdrain virus were 10~(-2.3) and 10~(-2.1).The infected test with the rats showed that the virus was pathogentic.At the same time,the sequence analysis indicated that the total length of HA gene was 1 702 bp and the homology with the virus in civil and abroad was 90.8% to 99%. |
| Keywords: | Swine influenza virus RT-PCR EID_(50) TICD_(50) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |