| 甲草胺在玉米籽粒上的QuEChERS-HPLC残留分析方法探究 |
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| 引用本文： | 汪玉,高子登,张剑南,胡树文.甲草胺在玉米籽粒上的QuEChERS-HPLC残留分析方法探究[J].中国农学通报,2014,30(31):295-299. |
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| 作者姓名： | 汪玉 高子登 张剑南 胡树文 |
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| 作者单位： | 中国农业大学资源与环境学院环境科学与工程系,中国农业大学资源与环境学院环境工程与科学系,中国农业大学资源与环境学院环境科学与工程系,中国农业大学资源与环境学院环境科学与工程系 |
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| 基金项目： | 农业部公益性行业专项“一次性施肥技术及推广示范”(201303103)；科技部“十二五”科技支撑计划“环境友好型高分子膜材料研发”(2011BAD11B02) |
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| 摘 要： | 建立了一种简单、快速、环保的甲草胺在玉米籽粒中的QuEChERS-HPLC残留分析方法。样品用极少量的乙腈提取，用N-丙基乙二胺（PSA）和石墨化炭黑（GCB）净化，高效液相色谱（紫外检测器）检测甲草胺浓度。色谱柱为C18反相柱(250 mm×4.6 mm×5.0μm)，流动相乙腈和水（70:30）溶液。对玉米中的甲草胺进行不同水平的添加回收率实验，在考察浓度范围内线性关系良好(R2≥0.994)；在0.2~10 mg/kg添加水平内，平均加标回收率为96.7%~113.8%，相对标准偏差（RSD）为3.2%~15.3%；检出限和定量限分别为0.015 mg/kg、0.05 mg/kg。该方法的精密度、灵敏度和准确度符合农药残留分析的要求。
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| 关 键 词： | 霜害温度指标 霜害温度指标 |
| 收稿时间： | 5/6/2014 12:00:00 AM |
| 修稿时间： | 5/6/2014 12:00:00 AM |
QuEChERS-HPLC Residue Analysis Method of Alachlor in Corn |
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| Institution: | Wang Yu, Gao Zideng, Zhang Jiannan, Lv Lianqi, Liu Dongmei, Hu Shuwen (1Department of Environmental Sciences ＆ Engineering, College of Resources ＆ Environmental Sciences, China Agricultural University, Beijing 100193; 2Rencheng District of Jining Municipal Bureau of Agricultural, Jining Shandong 272100) |
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| Abstract: | A simple, fast, and environmental-friendly determination method of alachlor residue in corn was established using QuEChERS- HPLC equipped with UV detector. Samples were extracted by a little acetonitrile, purified by N- propyl ethylenediamine（PSA） and graphitized carbon black（GCB）. The concentration of alachlor was detected by HPLC-UV. The analytical column was C18（250 mm×4.6 mm×5.0 μm）and the mobile phase was acetonitrile mixed with water（70：30 V/V）. With a significant linear relation （R^2≥0.994） of standard samples measured by this method, the average recovery rates of different alachlor doses（0.2-10 mg/kg） were 96.7%-113.8% and RSD were 3.2%-15.3%. The limit of detection and the limit of quantitation were 0.015 mg/kg and 0.05 mg/kg respectively. The accuracy, sensitivity and precision of this method can meet the requirement of pesticide residue analysis. |
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| Keywords: | QuEChERS alachlor corn HPLC |
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