| 可溶性玉米须多糖的提取与纯化 |
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| 引用本文： | 王在贵,刘朝良,吴晓峰,方杨武.可溶性玉米须多糖的提取与纯化[J].中国饲料,2010(9). |
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| 作者姓名： | 王在贵 刘朝良 吴晓峰 方杨武 |
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| 作者单位： | 安徽农业大学生命科学学院 |
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| 基金项目： | 安徽高校省级自然科学研究重点项目，合肥市农业科研计划项目，动物营养学国家重点实验室开放课题，安徽农业大学大学生创新基金项目 |
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| 摘 要： | 本试验采用水提醇沉法提取玉米须多糖,在不同的煎煮时间、提取温度、水料比条件下,探索最佳提取条件。并用不同体积的乙醇沉淀多糖,得出最佳醇沉体积比。水提后的玉米须残渣用β-葡聚糖酶和木聚糖酶酶解提取,比较用不同的酶量酶解条件下的最适提取条件,提取的粗多糖用三氯乙酸(TCA)法除多糖蛋白,用紫外扫描检验蛋白是否除尽。结果表明,玉米须多糖提取的最佳条件为:80min、80℃、18倍的水料比、2.5倍体积乙醇沉淀效果最佳;3%浓度的TCA溶液除蛋白质效果最好;通过紫外扫描检测纯化样品表明糖结合蛋白基本除尽。
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| 关 键 词： | 玉米须 多糖 提取 纯化 |
Extraction and purification of soluble polysaccharide from corn silk |
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| Wang Zaigui,Liu Chaoliang,Wu Xiaofeng,et al..Extraction and purification of soluble polysaccharide from corn silk[J].China Feed,2010(9). |
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| Authors: | Wang Zaigui Liu Chaoliang Wu Xiaofeng |
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| Abstract: | In this experiment,the factors influencing extraction of soluble polysaccharide extraction with water from corn silk were studied and the concentration of polysaccharide was determined by Sulfuric-phenol.The factors such as extracting time,temperature,extracting volume times of water and depositing volume times of ethanol were mainly researched.Then the polysaccharide was purifid by the method of TCA.In conclusion,the opitumum extraction condition of polysaccharide from corn silk was that the extraction tim... |
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| Keywords: | corn silk polysaccharide extraction purification |
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