| T1083替换融合质粒载体pGBKT7-TS转入酵母的自激活实验 |
| |
| 引用本文： | 袁亮,纪耀坤,张伟彬.T1083替换融合质粒载体pGBKT7-TS转入酵母的自激活实验[J].安徽农业科学,2010,38(14):7189-7190,7203. |
| |
| 作者姓名： | 袁亮 纪耀坤 张伟彬 |
| |
| 作者单位： | 商丘职业技术学院,河南商丘,476100;商丘职业技术学院,河南商丘,476100;商丘职业技术学院,河南商丘,476100 |
| |
| 摘 要： | 目的]研究T1083替换融合质粒载体pGBKT7-TS转入酵母的自激活实验,探讨其表达产物是否可作为诱饵进行酵母双杂交筛选。方法]将T1083替换突变BD融合质粒载体pGBKT7-TS转入酵母后,进行自激活和蛋白表达毒性检测试验。结果]该片段表达产物无自激活特性且对酵母细胞无毒性,可以作为诱饵进行酵母双杂交筛选。结论]为进一步研究NtKRP尾部T1083替换对NtKRP与靶物质结合的影响奠定了实验基础。
|
| 关 键 词： | pGBKT7-TS载体 酵母双杂交 诱饵载体 自激活作用 |
The Self-activated Experimental of T1083 Substitution Mutation Vector pGBKT7-TS in Yeast |
| |
| YUAN Liang et al.The Self-activated Experimental of T1083 Substitution Mutation Vector pGBKT7-TS in Yeast[J].Journal of Anhui Agricultural Sciences,2010,38(14):7189-7190,7203. |
| |
| Authors: | YUAN Liang |
| |
| Institution: | YUAN Liang et al (Shangqiu Vocational Technology Institute,Shangqiu,Henan 476100) |
| |
| Abstract: | Objective]The research aimed to study the self-activation test of inverting T1083 substitution mutation BD fusion vector pGBKT7-TS into yeast,and discuss whether its expression product could be used as bait for further two-hybrid screening. Method]T1083 substitution mutation BD fusion vector pGBKT7-TS was inverted into yeast to make the self-activation and protein expression toxic detection test. Result]This expres-sion product of the fragment was inactive and had no toxic to yeast cell. It could be used... |
| |
| Keywords: | pGBKT7-TS vector Yeast two-hybrid Bait vector Self-activation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
